| Texto completo | |
| Autor(es): |
da Silva, Marcelo J.
[1]
;
Fogarin Destro, Raquel
[1]
;
Gazoni, Thiago
[1]
;
Narimatsu, Hideki
[1]
;
Pereira dos Santos, Paulo S.
[1]
;
Haddad, Celio F. B.
[2, 3]
;
Parise-Maltempi, Patricia P.
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Biol, Ave 24A, 1515, BR-13506900 Rio Claro, SP - Brazil
[2] Univ Estadual Paulista UNESP, Dept Zool, Rio Claro - Brazil
[3] Univ Estadual Paulista UNESP, Ctr Aquicultura, Inst Biociencias, Rio Claro - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Cytogenetic and Genome Research; v. 160, n. 3, p. 141-147, APR 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Most eukaryotic genomes contain substantial portions of repetitive DNA sequences. These are located primarily in highly compacted heterochromatin and, in many cases, are one of the most abundant components of the sex chromosomes. In this sense, the anuranProceratophrys boieirepresents an interesting model for analyses on repetitive sequences by means of cytogenetic techniques, since it has a karyotype with large blocks of heterochromatin and a ZZ/ZW sex chromosome system. The present study describes, for the first time, families of satellite DNA (satDNA) in the frogP. boiei. Its genome size was estimated at 1.6 Gb, of which 41% correspond to repetitive sequences, including satDNAs, rDNAs, transposable elements, and other elements characterized as non-repetitive. The satDNAs were mapped by FISH in the centromeric and pericentromeric regions of all chromosomes, suggesting a possible involvement of these sequences in centromere function. SatDNAs are also present in the W sex chromosome, occupying the entire heterochromatic area, indicating a probable contribution of this class of repetitive DNA to the differentiation of the sex chromosomes in this species. This study is a valuable contribution to the existing knowledge on repetitive sequences in amphibians. We show the presence of repetitive DNAs, especially satDNAs, in the genome ofP. boieithat might be of relevance in genome organization and regulation, setting the stage for a deeper functional genome analysis ofProceratophrys. (AU) | |
| Processo FAPESP: | 13/50741-7 - Diversidade e conservação dos anfíbios brasileiros |
| Beneficiário: | Célio Fernando Baptista Haddad |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Temático |