Increased P3HB Accumulation Capacity ofMethylorubrumsp. in Response to Discontinuous Methanol Addition

Autor(es)

Bispo Cardoso, Leticia Oliveira ^{[1, 2, 3]} ; Karolski, Bruno ^{[2, 3]} ; Gracioso, Louise Hase ^{[1, 2, 3]} ; Oller do Nascimento, Claudio Augusto ^{[1, 2, 3]} ; Perpetuo, Elen Aquino ^{[2, 3, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Chem Engn Dept POLI USP, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Res Ctr Gas Innovat RCGI POLI USP, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Environm Res & Educ Ctr CEPEMA POLI USP, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Inst Marine Sci IMar UNIFESP, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 192, n. 3 JUL 2020.
• Citações Web of Science: 0

Resumo

An alternative for non-biodegradable oil-based plastics has been the focus of many researchers throughout the years. Polyhydroxyalkanoates (PHAs) are potential substitutes due to their biodegradable characteristic and diversity of monomers that allow different biopolymer compositions and physical-chemical properties suitable for a variety of applications. The most well-known biopolymer from this class, poly(3-hydroxybutyrate) (P3HB), is already produced industrially, but its final price cannot compete with the oil-based plastics. As a low-volume high-value bioproduct, P3HB must be produced through a cheap and abundant feedstock, with high productivity and a feasible purity process in order to become an economically attractive bioproduct. In this scenario, we report a methylotrophic strain isolated from an estuarine contaminated site identified asMethylorubrumsp. highly tolerant to methanol and with great accumulation capacity of 60% (CDW) in 48 h through a simple strategy of batch fermentation with discontinuous methanol addition that could help lower P3HB's processing costs and final price. (AU)

• Processo FAPESP: 17/17438-0 - Mitigação de CO2 e CH4 através do uso de consórcio microbiano (algas e bactérias)
• Beneficiário: Louise Hase Gracioso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/22790-7 - Avaliação do potencial da produção de biopolímeros por bactérias metilotróficas
• Beneficiário: Elen Aquino Perpetuo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular