Increased P3HB Accumulation Capacity ofMethylorubrumsp. in Response to Discontinuous Methanol Addition

Author(s)

Bispo Cardoso, Leticia Oliveira ^{[1, 2, 3]} ; Karolski, Bruno ^{[2, 3]} ; Gracioso, Louise Hase ^{[1, 2, 3]} ; Oller do Nascimento, Claudio Augusto ^{[1, 2, 3]} ; Perpetuo, Elen Aquino ^{[2, 3, 4]}

Affiliation

^[1] Univ Sao Paulo, Chem Engn Dept POLI USP, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Res Ctr Gas Innovat RCGI POLI USP, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Environm Res & Educ Ctr CEPEMA POLI USP, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Inst Marine Sci IMar UNIFESP, Sao Paulo - Brazil

• Document type: Journal article
• Source: Applied Biochemistry and Biotechnology; v. 192, n. 3 JUL 2020.
• Web of Science Citations: 0

Abstract

An alternative for non-biodegradable oil-based plastics has been the focus of many researchers throughout the years. Polyhydroxyalkanoates (PHAs) are potential substitutes due to their biodegradable characteristic and diversity of monomers that allow different biopolymer compositions and physical-chemical properties suitable for a variety of applications. The most well-known biopolymer from this class, poly(3-hydroxybutyrate) (P3HB), is already produced industrially, but its final price cannot compete with the oil-based plastics. As a low-volume high-value bioproduct, P3HB must be produced through a cheap and abundant feedstock, with high productivity and a feasible purity process in order to become an economically attractive bioproduct. In this scenario, we report a methylotrophic strain isolated from an estuarine contaminated site identified asMethylorubrumsp. highly tolerant to methanol and with great accumulation capacity of 60% (CDW) in 48 h through a simple strategy of batch fermentation with discontinuous methanol addition that could help lower P3HB's processing costs and final price. (AU)

• FAPESP's process: 17/17438-0 - Mitigation of CO2 and CH4 using microbial consortium (algae and bacteria)
• Grantee: Louise Hase Gracioso
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/22790-7 - Evaluation of the potential of the production of biopolymers by methylotrophic bacteria
• Grantee: Elen Aquino Perpetuo
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants