Lymphocyte responses to Mycobacterium tuberculosis and Mycobacterium bovis are similar between BCG-vaccinated patients with cystic fibrosis and healthy controls

Autor(es)

Mauch, Renan M. ^[1] ; Alves, Paulo Cesar M. ^[1] ; Levy, Carlos E. ^[2] ; Ribeiro, Jose D. ^[1] ; Ribeiro, Antonio F. ^[1] ; Hoiby, Niels ^[3] ; Nolasco da Silva, Marcos T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Ctr Invest Pediat, Sch Med Sci, Rua Tessalia Vieira Camargo 126, FCM 04, BR-13083887 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Sch Med Sci, Dept Clin Pathol, Campinas, SP - Brazil

^[3] Copenhagen Univ Hosp, Clin Microbiol Dept, Rigshosp, Copenhagen - Denmark

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cystic Fibrosis; v. 19, n. 4, p. 575-579, JUL 2020.
• Citações Web of Science: 0

Resumo

Background: The low rate of nontuberculous mycobacteria (NTM) among Brazilian patients with cystic fibrosis (CF) may be due to cross-reactive Bacille Calmette-Guerin (BCG) vaccination. In the present pilot study, we aimed to compare the lymphocyte responses against Mycobacterium tuberculosis (Mtb) and Mycobacterium bovis (BCG) in BCG-vaccinated CF patients and healthy controls. Methods: The lymphocyte responses of CF patients (n = 10) and healthy controls (n = 10) were assessed in terms of lymphocyte proliferation index (LPI), using flow cytometry. Median rates of each cell subtype - CD4, CD8, gamma delta T cells and CD19 (B) cells - were also determined. Results: Median LPIs (CF vs. controls) were 22.9% vs. 13.0% (p = 0.481) and 23.1% vs. 17.6% (p = 0.481), upon stimulation with Mtb and BCG, respectively. Both groups had a predominant CD4 T cell response to Mtb (median rate = 82.5% vs. 79.7%; p = 0.796) and BCG (LPI = 84.3% vs. 83.0%; p = 0.853), which were significantly higher than the CD8, CD19 and gamma delta responses within both groups. CF patients tended to have a higher CD8 T cell response upon stimulation with the phytohemagglutinin mitogen than healthy controls (median rate = 42.8% vs. 31.7%, p = 0.075). Conclusion: The responses of BCG-vaccinated CF patients to Mtb and BCG are at least similar to those of healthy individuals. These are probably memory responses elicited by the BCG vaccination, which can cross-react with NTM and may explain the low frequency of NTM lung infection in our CF center. (C) 2020 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 19/08598-9 - Resposta imune inata e adaptativa na persistência da infecção pulmonar por Pseudomonas aeruginosa e micobactérias na fibrose cística
• Beneficiário: Marcos Tadeu Nolasco da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/10614-0 - Resposta imune inata e adaptativa na persistência da infecção pulmonar por Pseudomonas aeruginosa produtora de biofilme na fibrose cística
• Beneficiário: Renan Marrichi Mauch
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado