Arginine-modified chitosan complexed with liposome systems for plasmid DNA delivery

Autor(es)

Garcia, Bianca B. M. ^{[1, 2]} ; Mertins, Omar ^[1] ; da Silva, Emerson R. ^[1] ; Mathews, Patrick D. ^[1] ; Han, Sang W. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biophys, Escola Paulista Med, Rua Botucatu 862, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Interdisciplinary Ctr GeneTherapy, Escola Paulista Med, BR-04044010 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 193, SEP 2020.
• Citações Web of Science: 1

Resumo

In order to make more efficient chitosan-based nanoparticles for transfection in physiological condition, chitosomes composed of chitosan modified with arginine and complexed with DOTAP/DOPE lipids are synthesized (named chitosomes) by reverse phase evaporation technique. Structure analyses of chitosomes with or without plasmid DNA (pDNA) are performed by electrophoresis, zeta potential, dynamic light scattering, small angle X-ray scattering and isothermal titration calorimetry, and transfection efficiency and cytotoxicity are performed in HEK293 T cells. Chitosomes have a positive surface charge ((X) over bar = 52mV) with an average size of 116 nm, and interaction with pDNA are favored thermodynamically and do not suffer aggregation significantly. In our experimental conditions, the transfection efficiency average reaches 86% +/- 3, while the Lipofectamine (R) reaches 87% +/- 5 in vitro. Cytotoxicity of chitosomes are tolerable. Structural analyses show that that chitosomes-pDNA complexes appear to have multilamellar vesicle structures hosting pDNA in-between bilayers which favor interaction with cell membrane and delivery of pDNA. Results show that synthesized chitosomes are promising carriers for gene delivery. (AU)

• Processo FAPESP: 16/13368-4 - Sistemas nanoestruturados: de modelos biomiméticos de membranas a carreadores de bioativos
• Beneficiário: Karin Do Amaral Riske
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/23948-5 - Aperfeiçoamento das propriedades do polissacarídeo quitosana para aplicação em lipossomas e vesículas gigantes
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• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/20206-8 - Modulação de monócitos, macrófagos e pericitos pelos genes dos fatores estimuladores de colônia para tratamento de isquemia de membros em modelo murino
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• Modalidade de apoio: Auxílio à Pesquisa - Temático