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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Arginine-modified chitosan complexed with liposome systems for plasmid DNA delivery

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Garcia, Bianca B. M. [1, 2] ; Mertins, Omar [1] ; da Silva, Emerson R. [1] ; Mathews, Patrick D. [1] ; Han, Sang W. [1, 2]
Total Authors: 5
[1] Univ Fed Sao Paulo, Dept Biophys, Escola Paulista Med, Rua Botucatu 862, BR-04023062 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Interdisciplinary Ctr GeneTherapy, Escola Paulista Med, BR-04044010 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 1

In order to make more efficient chitosan-based nanoparticles for transfection in physiological condition, chitosomes composed of chitosan modified with arginine and complexed with DOTAP/DOPE lipids are synthesized (named chitosomes) by reverse phase evaporation technique. Structure analyses of chitosomes with or without plasmid DNA (pDNA) are performed by electrophoresis, zeta potential, dynamic light scattering, small angle X-ray scattering and isothermal titration calorimetry, and transfection efficiency and cytotoxicity are performed in HEK293 T cells. Chitosomes have a positive surface charge ((X) over bar = 52mV) with an average size of 116 nm, and interaction with pDNA are favored thermodynamically and do not suffer aggregation significantly. In our experimental conditions, the transfection efficiency average reaches 86% +/- 3, while the Lipofectamine (R) reaches 87% +/- 5 in vitro. Cytotoxicity of chitosomes are tolerable. Structural analyses show that that chitosomes-pDNA complexes appear to have multilamellar vesicle structures hosting pDNA in-between bilayers which favor interaction with cell membrane and delivery of pDNA. Results show that synthesized chitosomes are promising carriers for gene delivery. (AU)

FAPESP's process: 15/23948-5 - Improvement of the polysaccharide chitosan properties for its application in liposomes and giant vesicles
Grantee:Omar Mertins
Support type: Regular Research Grants
FAPESP's process: 15/20206-8 - Modulation of monocytes, macrophages and pericytes by the colony stimulating factor genes to treat murine limb ischemia
Grantee:Sang Won Han
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/13368-4 - Nanostructured systems: from membrane biomimetic models to carriers of bioactives
Grantee:Karin Do Amaral Riske
Support type: Research Projects - Thematic Grants