Enhanced chitosan effects on cell membrane models made with lipid raft monolayers

Autor(es)

Pereira, Andressa R. ^[1] ; Fiamingo, Anderson ^{[1, 2]} ; Pedro, Rafael de O. ^[1] ; Campana-Filho, Sergio P. ^[2] ; Miranda, Paulo B. ^[1] ; Oliveira Jr, Osvaldo N.

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Chem, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 193, SEP 2020.
• Citações Web of Science: 0

Resumo

Langmuir monolayers have been used as cell membrane models, where lipid composition is normally varied to mimic distinct types of membranes. For eukaryotic membranes, for instance, rather than using only zwitterionic phospholipids there is now a trend to employ mixtures to simulate the lipid rafts known to be relevant for various cellular processes. In this study, we demonstrate that effects from chitosans on Langmuir monolayers are considerably higher if lipid raft compositions (ternary mixtures of dipalmitoyl phosphatidyl choline (DPPC), sphingomyelin (SM) and cholesterol) are used. Significantly, measurable effects on the surface pressure isotherms start at 10(-6) mg mL(-1) for chitosans in lipid rafts, to be compared with 10(-2) mg mL(-1) for neat dipalmitoyl phosphatidylcholine (DPPC). This applies to both a commercial chitosan and chitosans soluble at physiological pH. Incorporation of these chitosans in the raft monolayers was confirmed in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) experiments, where both the tail groups and headgroups were found to interact with chitosan. Since the effects on membrane models may be observed at such small concentrations for chitosans and probably other molecules, some studies may have to be revisited where neat phospholipids should be replaced by lipid raft compositions. (AU)

• Processo FAPESP: 17/03503-4 - Interação molecular entre quitosanas e modelos de membrana celular investigada por espectroscopia vibracional não linear
• Beneficiário: Rafael de Oliveira Pedro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/00878-0 - Estudo de jangadas lipídicas com a interação entre modelos de membrana e quitosana e colesterol oxidase
• Beneficiário: Andressa Ribeiro Pereira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/09720-4 - Utilização de modelos de membrana para avaliar a biodegradabilidade de quitosanas
• Beneficiário: Anderson Fiamingo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado