Sugarcane Syrup Improves Xylitol Bioproduction from Sugarcane Bagasse and Straw Hemicellulosic Hydrolysate

Autor(es)

Hernandez-Perez, Andres Felipe ^[1] ; Chaves-Villamil, Alejandra Camila ^[2] ; de Arruda, Priscila Vaz ^[3] ; dos Santos, Julio Cesar ^[1] ; Felipe, Maria das Gracas de Almeida ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Engn Lorena, Dept Biotecnol, BR-12602810 Lorena, SP - Brazil

^[2] Univ Distrital Francisco Jose de Caldas, Fac Medio Ambiente & Recursos Nat, Bogota, DC - Colombia

^[3] Univ Tecnol Fed Parana, Toledo, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WASTE AND BIOMASS VALORIZATION; v. 11, n. 8, p. 4215-4224, AUG 2020.
• Citações Web of Science: 1

Resumo

To evaluate the supplementation of the sugarcane bagasse and straw hemicellulosic hydrolysate with sugarcane syrup, which is a sucrose-rich co-product, for xylitol production byCandida guilliermondiiFTI 20037. Recently, we demonstrated that sucrose supplementation to sugarcane straw hemicellulosic hydrolysate favoured this bioprocess. A mixture 1:1 of sugarcane bagasse and straw was submitted to dilute-acid hydrolysis with H(2)SO(4)to obtain the hemicellulosic hydrolysate, which was concentrated, detoxified and autoclaved. Three sugarcane syrup concentrations (0.83, 2.50 and 3.33% v/v) were added to the hemicellulosic hydrolysate, which was also supplemented with rice bran extract, (NH4)(2)SO4, CaCl2 center dot 2H(2)O. Next, a full factorial design was performed to assess the replacement of conventional nutrients by sugarcane syrup. Fermentations were performed in 125 mL Erlenmeyer flasks containing 50 mL of fermentation medium, for 48 h at 30 degrees C and 200 rpm. Improvements of 16.85% and 12.24% in xylose uptake rate (1.04 +/- 0.01 g L-1 h(-1)) and xylitol volumetric productivity (0.55 +/- 0.02 g L-1 h(-1)), respectively, were achieved with syrup supplementation (2.50% v/v), which led to a final xylitol titer of 26.19 +/- 0.02 g L-1. Conventionally employed nutrients (rice bran extract, (NH4)(2)SO(4)and CaCl2 center dot 2H(2)O) did not have significant influence on xylitol production when sugarcane syrup was added to the fermentation medium. Sugarcane syrup as a nutritional supplement improved xylitol production from a mixture of bagasse and straw, becoming an alternative to reduce costs in the formulation of the fermentation medium and contributing to the integration of this bioprocess in a sugarcane biorefinery. {[}GRAPHICS] . (AU)

• Processo FAPESP: 16/22179-0 - Melhorias no bioprocesso de produção de xilitol no conceito de biorrefinaria para diversificação do portfólio de produtos do setor sucroalcooleiro
• Beneficiário: Maria das Graças de Almeida Felipe
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/05971-2 - Produção biotecnológica de xilitol a partir da mistura de bagaço e palha de cana-de-açúcar com aproveitamento do melaço: otimização das condições de cultivo e engenharia da via metabólica
• Beneficiário: Andrés Felipe Hernández Pérez
• Modalidade de apoio: Bolsas no Brasil - Doutorado