Sugarcane Syrup Improves Xylitol Bioproduction from Sugarcane Bagasse and Straw Hemicellulosic Hydrolysate

Author(s)

Hernandez-Perez, Andres Felipe ^[1] ; Chaves-Villamil, Alejandra Camila ^[2] ; de Arruda, Priscila Vaz ^[3] ; dos Santos, Julio Cesar ^[1] ; Felipe, Maria das Gracas de Almeida ^[1]

Affiliation

^[1] Univ Sao Paulo, Escola Engn Lorena, Dept Biotecnol, BR-12602810 Lorena, SP - Brazil

^[2] Univ Distrital Francisco Jose de Caldas, Fac Medio Ambiente & Recursos Nat, Bogota, DC - Colombia

^[3] Univ Tecnol Fed Parana, Toledo, PR - Brazil

• Document type: Journal article
• Source: WASTE AND BIOMASS VALORIZATION; v. 11, n. 8, p. 4215-4224, AUG 2020.
• Web of Science Citations: 1

Abstract

To evaluate the supplementation of the sugarcane bagasse and straw hemicellulosic hydrolysate with sugarcane syrup, which is a sucrose-rich co-product, for xylitol production byCandida guilliermondiiFTI 20037. Recently, we demonstrated that sucrose supplementation to sugarcane straw hemicellulosic hydrolysate favoured this bioprocess. A mixture 1:1 of sugarcane bagasse and straw was submitted to dilute-acid hydrolysis with H(2)SO(4)to obtain the hemicellulosic hydrolysate, which was concentrated, detoxified and autoclaved. Three sugarcane syrup concentrations (0.83, 2.50 and 3.33% v/v) were added to the hemicellulosic hydrolysate, which was also supplemented with rice bran extract, (NH4)(2)SO4, CaCl2 center dot 2H(2)O. Next, a full factorial design was performed to assess the replacement of conventional nutrients by sugarcane syrup. Fermentations were performed in 125 mL Erlenmeyer flasks containing 50 mL of fermentation medium, for 48 h at 30 degrees C and 200 rpm. Improvements of 16.85% and 12.24% in xylose uptake rate (1.04 +/- 0.01 g L-1 h(-1)) and xylitol volumetric productivity (0.55 +/- 0.02 g L-1 h(-1)), respectively, were achieved with syrup supplementation (2.50% v/v), which led to a final xylitol titer of 26.19 +/- 0.02 g L-1. Conventionally employed nutrients (rice bran extract, (NH4)(2)SO(4)and CaCl2 center dot 2H(2)O) did not have significant influence on xylitol production when sugarcane syrup was added to the fermentation medium. Sugarcane syrup as a nutritional supplement improved xylitol production from a mixture of bagasse and straw, becoming an alternative to reduce costs in the formulation of the fermentation medium and contributing to the integration of this bioprocess in a sugarcane biorefinery. {[}GRAPHICS] . (AU)

• FAPESP's process: 16/22179-0 - Improvements of xylitol production bioprocess in the biorefinery concept for diversification of product portfolio of sugar-and-alcohol sector
• Grantee: Maria das Graças de Almeida Felipe
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/05971-2 - Biotechnological production of xylitol from the mixture of sugarcane bagasse and straw with utilization of molasses: optimization of cultivation conditions and engineering of the metabolic pathway
• Grantee: Andrés Felipe Hernández Pérez
• Support Opportunities: Scholarships in Brazil - Doctorate