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Improvements of xylitol production bioprocess in the biorefinery concept for diversification of product portfolio of SUGAR-AND-ALCOHOL sector

Grant number: 16/22179-0
Support type:Regular Research Grants
Duration: February 01, 2017 - July 31, 2019
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Maria das Graças de Almeida Felipe
Grantee:Maria das Graças de Almeida Felipe
Home Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil
Assoc. researchers:Fernando Segato ; Júlio César dos Santos ; Silvio Silvério da Silva ; Talita Ferreira Marques da Silva Fernandes ; Valdeir Arantes


Different approaches will be applied to improve the technical and economicfeasibility of xylitol production bioprocess from a mixture of sugarcane bagasse and straw inthe biorefinery concept for the sugar-alcohol sector. One of the aims is to increase thecapacity of the yeast Candida guilliermondii FTI 20037 to bioconvert the xylose present inthe hemicellulose to xylitol by optimizing the culture conditions and metabolic pathwayengineering. These approaches are based on important progresses achieved at "Escola deEngenharia de Lorena - Universidade de São Paulo", employing this yeast and differentbiomasses, mainly sugarcane bagasse, as well as on a recent research (FAPESP No.2013/27142-0) in which sugarcane straw was the subject of study. Oxygen supplied, initialconcentrations of xylose, sucrose and inoculum will be studied, followed by evaluation ofnutritional supplementation of the hydrolysate with sugarcane molasses as a substitution ofsucrose and an additional source of nitrogenous compounds and minerals. Regarding themetabolic pathway engineering, C. guilliermondii FTI 20037 will be sequentially transformedby attenuation of xylitol dehydrogenase, over-expression of xylose reductase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and attenuation ofphosphoglucoisomerase. The third approach corresponds to the evaluation of the feasibility ofusing the cellulignin residue from this bioprocess for nanocellulose production, a high-addedvalue biomaterial with application ranging from civil construction to medicine. It is expectedthat the outcomes of this project will contribute to product diversification of the sugaralcoholsector creating opportunities for integral utilization of sugarcane bagasse and straw, aswell as of molasses, in the biorefinery concept. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HERNANDEZ-PEREZ, ANDRES FELIPE; CHAVES-VILLAMIL, ALEJANDRA CAMILA; DE ARRUDA, PRISCILA VAZ; DOS SANTOS, JULIO CESAR; FELIPE, MARIA DAS GRACAS DE ALMEIDA. Sugarcane Syrup Improves Xylitol Bioproduction from Sugarcane Bagasse and Straw Hemicellulosic Hydrolysate. WASTE AND BIOMASS VALORIZATION, v. 11, n. 8, p. 4215-4224, AUG 2020. Web of Science Citations: 1.
HERNANDEZ-PEREZ, ANDRES FELIPE; DE ARRUDA, PRISCILA VAZ; SENE, LUCIANE; DA SILVA, SILVIO SILVERIO; CHANDEL, ANUJ KUMAR; DE ALMEIDA FELIPE, MARIA DAS GRACAS. Xylitol bioproduction: state-of-the-art, industrial paradigm shift, and opportunities for integrated biorefineries. CRITICAL REVIEWS IN BIOTECHNOLOGY, v. 39, n. 7 JULY 2019. Web of Science Citations: 0.

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