| Full text | |
| Author(s): |
Queiroz, Sarah S.
;
Oliva, Bianca
;
Silva, Tatiane F.
;
Segato, Fernando
;
Felipe, Maria G. A.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | Applied Microbiology and Biotechnology; v. N/A, p. 20-pg., 2022-06-16. |
| Abstract | |
The transport of substrates across the cell membrane plays an essential role in nutrient assimilation by yeasts. The establishment of an efficient microbial cell factory, based on the maximum use of available carbon sources, can generate new technologies that allow the full use of lignocellulosic constituents. These technologies are of interest because they could promote the formation of added-value products with economic feasibility. In silico analyses were performed to investigate gene sequences capable of encoding xylose transporter proteins in the Candida tropicalis genome. The current study identified 11 putative transport proteins that have not yet been functionally characterized. A phylogenetic tree highlighted the potential C. tropicalis xylose-transporter proteins CtXUT1, CtXUT4, CtSTL1, CtSTL2, and CtGXT2, which were homologous to previously characterized and reported xylose transporters. Their expression was quantified through real-time qPCR at defined times, determined through a kinetic analysis of the microbial growth curve in the absence/presence of glucose supplemented with xylose as the main carbon source. The results indicated different mRNA expression levels for each gene. CtXUT1 mRNA expression was only found in the absence of glucose in the medium. Maximum CtXUT1 expression was observed in intervals of the highest xylose consumption (21 to 36 h) that corresponded to consumption rates of 1.02 and 0.82 g/L/h in the formulated media, with xylose as the only carbon source and with glucose addition. These observations indicate that CtXUT1 is an important xylose transporter in C. tropicalis. (AU) | |
| FAPESP's process: | 19/22284-7 - Biochemical, functional characterization and application of polysaccharide lytic monoxygenases identified in transcriptome and secretoma studies of filamentous fungi cultivated in sugarcane bagasse |
| Grantee: | Fernando Segato |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |
| FAPESP's process: | 19/27092-9 - Xylitol-producing biorefinery from sugarcane: new strategies to make the biotechnological route viable |
| Grantee: | Maria das Graças de Almeida Felipe |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |
| FAPESP's process: | 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory |
| Grantee: | Fernando Segato |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Young Investigators Grants |
| FAPESP's process: | 16/22179-0 - Improvements of xylitol production bioprocess in the biorefinery concept for diversification of product portfolio of sugar-and-alcohol sector |
| Grantee: | Maria das Graças de Almeida Felipe |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/06663-8 - Biochemical and functional studies of cellobiohydrolases from basidiomycetes of white-rot fungi with biotechnological potential |
| Grantee: | Bianca Oliva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |