Transcriptional analysis of THP-1 cells infected with Leishmania infantum indicates no activation of the inflammasome platform

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Gatto, Mariana ^[1] ; Borim, Patricia Aparecida ^[1] ; Wolf, Ivan Rodrigo ^[2] ; da Cruz, Tais Fukuta ^[3] ; Ferreira Mota, Gustavo Augusto ^[4] ; Marques Braz, Aline Marcia ^[4] ; Amorim, Barbara Casella ^[1] ; Valente, Guilherme Targino ^[2] ; Golim, Marjorie de Assis ^[5] ; Venturini, James ^[6] ; Araujo Junior, Joao Pessoa ^[2] ; Pontillo, Alessandra ^[7] ; Sartori, Alexandrine ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Trop Dis Dept, Botucatu Med Sch, Botucatu, SP - Brazil

^[2] UNESP, Bioproc & Biotechnol Dept, Agron Sci Sch, Botucatu, SP - Brazil

^[3] UNESP, Microbiol & Immunol Dept, Biosci Inst, Botucatu, SP - Brazil

^[4] UNESP, Internal Med Dept, Botucatu Med Sch, Botucatu, SP - Brazil

^[5] UNESP, Flow Cytometry Lab, Botucatu Med Sch, Botucatu, SP - Brazil

^[6] Med Sch UFMS, Campo Grande, MS - Brazil

^[7] Sao Paulo Univ USP, Immunol Dept, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS Neglected Tropical Diseases; v. 14, n. 1 JAN 2020.
• Citações Web of Science: 0

Resumo

Leishmaniasis is caused by intracellular parasites transmitted to vertebrates by sandfly bites. Clinical manifestations include cutaneous, mucosal or visceral involvement depending upon the host immune response and the parasite species. To assure their survival inside macrophages, these parasites developed a plethora of highly successful strategies to manipulate various immune system pathways. Considering that inflammasome activation is critical for the establishment of a protective immune response in many parasite infections, in this study we determined the transcriptome of THP-1 cells after infection with L. infantum, with a particular focus on the inflammasome components. To this end, the human cell line THP-1, previously differentiated into macrophages by PMA treatment, was infected with L. infantum promastigotes. Differentiated THP-1 cells were also stimulated with LPS to be used as a comparative parameter. The gene expression signature was determined 8 hours after by RNA-seq technique. Infected or uninfected THP-1 cells were stimulated with nigericin (NIG) to measure active caspase-1 and TNF-alpha, IL-6 and IL-1 beta levels in culture supernatants after 8, 24 and 48 hours. L. infantum triggered a gene expression pattern more similar to non-infected THP-1 cells and very distinct from LPS-stimulated cells. Some of the most up-regulated genes in L. infantum-infected cells were CDC20, CSF1, RPS6KA1, CD36, DUSP2, DUSP5, DUSP7 and TNFAIP3. Some up-regulated GO terms in infected cells included cell coagulation, regulation of MAPK cascade, response to peptide hormone stimulus, negative regulation of transcription from RNA polymerase II promoter and nerve growth factor receptor signaling pathway. Infection was not able to induce the expression of genes associated with the inflammasome signaling pathway. This finding was confirmed by the absence of caspase-1 activation and IL-1 beta production after 8, 24 and 48 hours of infection. Our results indicate that L. infantum was unable to activate the inflammasomes during the initial interaction with THP-1 cells. (AU)

• Processo FAPESP: 14/04019-0 - Análise do padrão de resposta Th17, de células T regulatórias e do proteoma em pacientes com Doença de Chagas digestiva crônica
• Beneficiário: Angela Maria Victoriano de Campos Soares
• Modalidade de apoio: Auxílio à Pesquisa - Regular