Antimicrobial photodynamic therapy against metronidazole-resistant dental plaque bacteria

Autor(es)

de Paula Zago, Lucas Henrique ^[1] ; de Annunzio, Sarah Raquel ^[1] ; de Oliveira, Kleber Thiago ^[2] ; Barbugli, Paula Aboud ^{[1, 3]} ; Valdes, Belen Retamal ^[4] ; Feres, Magda ^[4] ; Fontana, Carla Raquel ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

^[3] Sao Paulo State Univ Unesp, Sch Dent, BR-14801903 Araraquara, SP - Brazil

^[4] Univ Guarulhos, Dent Res Div, Dept Periodontol, BR-07011040 Guarulhos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 209, AUG 2020.
• Citações Web of Science: 0

Resumo

The antimicrobial photodynamic therapy (aPDT) has stood out as an alternative and promising method of disinfection and has been exploited for the treatment of oral bacteria. In this study, we evaluate in vitro the action of aPDT, mediated by methylene blue, chlorin-e6, and curcumin against clinical subgingival plaques that were resistant to metronidazole. The sensitivity profile of the samples to metronidazole was analyzed by the agar dilution method. Cell viability in the planktonic and biofilm phase was assessed by CFU / mL. The composition of the biofilm was evaluated by the checkboard DNA-DNA Hibrydization technique. Photosensitizers internalization was qualitatively assessed by confocal fluorescence microscopy (CLSM). The aPDT mediated by the three photosensitizers tested was able to reduce the totality of the planktonic microbial load and partially reduce the biofilm samples. The analysis performed by CLSM showed that the photosensitizers used in the application of aPDT were able to permeate the interior of the biofilm. The aPDT has been shown to be useful in a supportive and effective approach to the treatment of periodontal disease. (AU)

• Processo FAPESP: 16/10310-5 - Avaliação da redução e resistência bacteriana via múltiplos ciclos de terapia fotodinâmica
• Beneficiário: Lucas Henrique de Paula Zago
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/09088-1 - Avaliação da terapia fotodinâmica contra biofilmes acneicos polimicrobianos: um estudo pré-clínico
• Beneficiário: Sarah Raquel de Annunzio
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 18/23015-7 - Terapia fotodinâmica antimicrobiana via irradiação em modo contínuo e chaveado contra Enterococcus faecalis e Cutibacterium acnes
• Beneficiário: Carla Raquel Fontana
• Modalidade de apoio: Auxílio à Pesquisa - Regular