Antimicrobial photodynamic therapy against metronidazole-resistant dental plaque bacteria

Author(s)

de Paula Zago, Lucas Henrique ^[1] ; de Annunzio, Sarah Raquel ^[1] ; de Oliveira, Kleber Thiago ^[2] ; Barbugli, Paula Aboud ^{[1, 3]} ; Valdes, Belen Retamal ^[4] ; Feres, Magda ^[4] ; Fontana, Carla Raquel ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

^[3] Sao Paulo State Univ Unesp, Sch Dent, BR-14801903 Araraquara, SP - Brazil

^[4] Univ Guarulhos, Dent Res Div, Dept Periodontol, BR-07011040 Guarulhos, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 209, AUG 2020.
• Web of Science Citations: 0

Abstract

The antimicrobial photodynamic therapy (aPDT) has stood out as an alternative and promising method of disinfection and has been exploited for the treatment of oral bacteria. In this study, we evaluate in vitro the action of aPDT, mediated by methylene blue, chlorin-e6, and curcumin against clinical subgingival plaques that were resistant to metronidazole. The sensitivity profile of the samples to metronidazole was analyzed by the agar dilution method. Cell viability in the planktonic and biofilm phase was assessed by CFU / mL. The composition of the biofilm was evaluated by the checkboard DNA-DNA Hibrydization technique. Photosensitizers internalization was qualitatively assessed by confocal fluorescence microscopy (CLSM). The aPDT mediated by the three photosensitizers tested was able to reduce the totality of the planktonic microbial load and partially reduce the biofilm samples. The analysis performed by CLSM showed that the photosensitizers used in the application of aPDT were able to permeate the interior of the biofilm. The aPDT has been shown to be useful in a supportive and effective approach to the treatment of periodontal disease. (AU)

• FAPESP's process: 16/10310-5 - Bacterial reduction and resistance after multiple cycles of photodynamic therapy
• Grantee: Lucas Henrique de Paula Zago
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/09088-1 - Assessment of photodynamic therapy against polymicrobial acne biofilms: a preclinical study
• Grantee: Sarah Raquel de Annunzio
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 18/23015-7 - Antimicrobial photodynamic therapy by continuous and switched mode irradiation against Enterococcus faecalis and Cutibacterium acnes
• Grantee: Carla Raquel Fontana
• Support Opportunities: Regular Research Grants