Antimicrobial photodynamic therapy by continuous and switched mode irradiation against Enterococcus faecalis and Cutibacterium acnes

• Grant number: 18/23015-7
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2019
• End date: May 31, 2021
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Carla Raquel Fontana
• Grantee: Carla Raquel Fontana
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Photodynamic therapy (PDT) has been used against bacterial, fungal and viral infections and recently demonstrated that there is no development of resistance by microorganisms. PDT consists of the activation of a photosensitizer (non-toxic dye) by light in an adequate wavelength, where the generation of reactive oxygen species (type I reaction) or energy transfer to the oxygen will lead to the production of oxygen singlet (type II reaction) causing cell death. Some of the photosensitizers used in the therapy are chlorins (reduced porphyrins), curcumin (natural dye extracted from Curcuma longa L. rhizomes) and methylene blue (cationic dye). PDT has been used as an adjuvant treatment in periodontics, implantology and endodontics to reduce the remaining microorganisms after mechanical treatment. The genus Enterococcus is part of the group of gram-positive, facultative anaerobic bacteria, residents of the gastrointestinal tract, vaginal and oral cavity. It is associated with endodontic infections and is resistant to intracanal drugs. In addition, TFD has been studied to be applied when root canal treatment fails where E. faecalis is able to survive as a single organism or as an important component of this flora. In addition to the genus Enterococcus, the genus Propionibacterium is also found in primary and persistent endodontic infections, and the species Cutibacterium acnes, formerly known as Propionibacterium acnes, is the most frequent species. Cutibacterium acnes (C. acnes) is a facultative anaerobic gram-positive bacterium that normally colonizes human sebaceous glands. Our rechearch group has observed that these two strains presented different responses in relation to light doses and the mode of irradiation also seems to be important. In this way, the present study aims to evaluate the phototoxicity of the irradiation in switched and continuous mode in PDT in strains of Enterococcus faecalis and Cutibacterium acnes, in suspension and in biofilm phase, using as photosensitizers methylene blue, chlorine and curcumin. (AU)

Scientific publications

(21)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; DE ANNUNZIO, SARAH RAQUEL; FRANCA, MARIA GLEIZIANE ARAUJO; SILVA, MARIA GORETTI DE VASCONCELOS; CAVALHEIRO, ALBERTO JOSE; FONTANA, CARLA RAQUEL; BRIGHENTI, FERNANDA LOURENCAO. In Vitro Evaluation of Photodynamic Activity of Plant Extracts from Senna Species against Microorganisms of Medical and Dental Interest. PHARMACEUTICS, v. 15, n. 1, p. 16-pg., 2023-01-01. (13/07600-3, 22/12828-2, 14/50926-0, 20/07110-0, 19/08375-0, 18/09088-1, 18/18440-0, 18/23015-7, 21/01191-0)

FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; SURUR, AMANDA KOBERSTAIN; BUZO, HELEN SORDI; BRIGHENTI, FERNANDA LOURENCAO; FONTANA, CARLA RAQUEL. Photodynamic therapy associated with nanomedicine strategies for treatment of human squamous cell carcinoma: A systematic review and meta-analysis. Nanomedicine-Nanotechnology Biology and Medicine, v. 40, p. 10-pg., 2022-02-01. (18/23015-7, 20/05169-7, 21/01191-0)

LOPERA SEPULVEDA, ALEX ARBEY; ARENAS VELASQUEZ, ANGELA MARIA; PATINO LINARES, IRWIN ALEXANDER; DE ALMEIDA, LETICIA; FONTANA, CARLA RAQUEL; GARCIA, CLAUDIA; SILVA GRAMINHA, MARCIA APARECIDA. Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis. Photodiagnosis and Photodynamic Therapy, v. 30, JUN 2020. (16/05345-4, 17/03552-5, 16/19289-9, 18/23015-7)

DE PAULA ZAGO, LUCAS HENRIQUE; DE ANNUNZIO, SARAH RAQUEL; DE OLIVEIRA, KLEBER THIAGO; BARBUGLI, PAULA ABOUD; VALDES, BELEN RETAMAL; FERES, MAGDA; FONTANA, CARLA RAQUEL. Antimicrobial photodynamic therapy against metronidazole-resistant dental plaque bacteria. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 209, AUG 2020. (16/10310-5, 18/09088-1, 13/07276-1, 18/23015-7)

DOS SANTOS, DANILO M.; DE ANNUNZIO, SARAH R.; CARMELLO, JULIANA C.; PAVARINA, ANA C.; FONTANA, CARLA R.; CORREA, DANIEL S.. Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment. ACS APPLIED BIO MATERIALS, DEC 2021. (18/22214-6, 18/23015-7, 17/20973-4, 18/09088-1)

FIORAMONTI CALIXTO, GIOVANA MARIA; DE ANNUNZIO, SARAH RAQUEL; VICTORELLI, FRANCESCA DAMIANI; FRADE, MARIA LUCIA; FERREIRA, PAULA SCANAVEZ; CHORILLI, MARLUS; FONTANA, CARLA RAQUEL. Chitosan-Based Drug Delivery Systems for Optimization of Photodynamic Therapy: a Review. AAPS PHARMSCITECH, v. 20, n. 7, OCT 2019. (18/23015-7, 18/09088-1)

VELASQUEZ, ANGELA M. A.; BARTLETT, PAULA J.; LINARES, IRWIN A. P.; PASSALACQUA, THAIS G.; TEODORO, DAPHNE D. L.; IMAMURA, KELY B.; VIRGILIO, STELA; TOSI, LUIZ R. O.; LEITE, ALINE DE LIMA; BUZALAF, MARILIA A. R.; et al. ew Insights into the Mechanism of Action of the Cyclopalladated Complex (CP2) in Leishmania: Calcium Dysregulation, Mitochondrial Dysfunction, and Cell Deat. Antimicrobial Agents and Chemotherapy, v. 66, n. 1, JAN 2022. (16/18191-5, 16/05345-4, 18/23015-7, 20/04415-4, 17/03552-5, 16/19289-9, 19/21661-1)

DE FREITAS, LAURA MARISE; LORENZON, ESTEBAN NICOLAS; CILLI, EDUARDO MAFFUD; DE OLIVEIRA, KLEBER THIAGO; FONTANA, CARLA RAQUEL; MANG, THOMAS S.. Photodynamic and peptide-based strategy to inhibit Gram-positive bacterial biofilm formation. BIOFOULING, v. 35, n. 7, p. 742-757, AUG 9 2019. (18/23015-7, 14/24581-5, 16/18378-8)

LINARES, IRWIN A. P.; URIA, MARICELY SANCHEZ; GRAMINHA, MARCIA A. S.; IGLESIAS, BERNARDO ALMEIDA; VELASQUEZ, ANGELA M. A.. Antileishmanial activity of tetra-cationic porphyrins with peripheral Pt(II) and Pd(II) complexes mediated by photodynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 42, p. 10-pg., 2023-06-13. (18/23015-7, 20/04415-4, 16/19289-9)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FRANCA, GABRIELA GOMES; DE ANNUNZIO, SARAH RAQUEL; KOPP, WILLIAN; FONTANA, CARLA RAQUEL; BRIGHENTI, FERNANDA LOURENCAO. Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms. PATHOGENS, v. 12, n. 2, p. 19-pg., 2023-02-01. (19/08375-0, 18/23015-7, 18/18440-0, 13/07276-1, 21/01191-0, 20/07110-0)

RICCIO, BRUNO VINCENZO FIOD; DO NASCIMENTO, ANDRE LUIZ CARNEIRO SOARES; MENEGUIN, ANDREIA BAGLIOTTI; RODERO, CAMILA FERNANDA; SANTOS, KAIO PINI; SABIO, RAFAEL MIGUEL; DE ANNUNZIO, SARAH RAQUEL; FONTANA, CARLA RAQUEL; BARUD, HERNANE DA SILVA; FERRARI, PRISCILEILA COLERATO; et al. Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation. PHARMACEUTICS, v. 14, n. 6, p. 21-pg., 2022-06-01. (19/19817-3, 18/09088-1, 18/23015-7, 18/25377-3, 18/23357-5, 19/00164-0)

SAMPAIO, LAIS SIMOES; DE ANNUNZIO, SARAH RAQUEL; DE FREITAS, LAURA MARISE; DANTAS, LIVIA OLIVEIRA; DE BONI, LEONARDO; DONATONI, MARIA CAROLINA; DE OLIVEIRA, KLEBER THIAGO; FONTANA, CARLA RAQUEL. Influence of light intensity and irradiation mode on methylene blue, chlorin-e6 and curcumin-mediated photodynamic therapy against Enterococcus faecalis. Photodiagnosis and Photodynamic Therapy, v. 31, p. 13-pg., 2020-09-01. (18/23015-7, 18/00106-7, 13/07276-1, 18/09088-1)

LORDELLO, VIRGINIA BARRETO; MENEGUIN, ANDREIA BAGLIOTTI; DE ANNUNZIO, SARAH RAQUEL; PIA TARANTO, MARIA; CHORILLI, MARLUS; FONTANA, CARLA RAQUEL; UMBELINO CAVALLINI, DANIELA CARDOSO. Orodispersible Film Loaded with Enterococcus faecium CRL183 Presents Anti-Candida albicans Biofilm Activity In Vitro. PHARMACEUTICS, v. 13, n. 7, JUL 2021. (18/09088-1, 18/23015-7)

POLLI SILVESTRE, AMANDA LETICIA; DI FILIPPO, LEONARDO DELELLO; BESEGATO, JOAO FELIPE; DE ANNUNZIO, SARAH RAQUEL; FURQUIM DE CAMARGO, BRUNA ALMEIDA; GOBBO DE MELO, PRISCILA BORGES; DE SOUZA RASTELLI, ALESSANDRA NARA; FONTANA, CARLA RAQUEL; CHORILLI, MARLUS. Current applications of drug delivery nanosystems associated with antimicrobial photodynamic therapy for oral infections. International Journal of Pharmaceutics, v. 592, JAN 5 2021. (18/23015-7, 18/23096-7, 18/18488-3, 18/09088-1)

DE SANTANA, WILLIAN MAX O. S.; CAETANO, BRUNO L.; DE ANNUNZIO, SARAH RAQUEL; PULCINELLI, SANDRA H.; MENAGER, CHRISTINE; FONTANA, CARLA RAQUEL; SANTILLI, V, CELSO. Conjugation of superparamagnetic iron oxide nanoparticles and curcumin photosensitizer to assist in photodynamic therapy. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 196, DEC 2020. (18/23015-7, 18/09088-1, 17/07822-7)

MOREIRA, VITOR PARTITE; DA SILVA MELA, MICHELE FERREIRA; DOS ANJOS, LUANA RIBEIRO; SARAIVA, LEONARDO FIGUEIREDO; VELASQUEZ, ANGELA M. ARENAS; KALABA, PREDRAG; FABISIKOVA, ANNA; CLEMENTINO, LEANDRO DA COSTA; AUFY, MOHAMMED; STUDENIK, CHRISTIAN; et al. Novel Selective and Low-Toxic Inhibitor of LmCPB2.8 Delta CTE (CPB) One Important Cysteine Protease for Leishmania Virulence. BIOMOLECULES, v. 12, n. 12, p. 21-pg., 2022-12-01. (18/00581-7, 20/04415-4, 16/19289-9, 17/03552-5, 18/23015-7, 21/02595-8)

DE ANNUNZIO, SARAH RAQUEL; SILVA COSTA, NATALIA CAROLINE; MEZZINA, RAFAELA DALBELLO; GRAMINHA, MARCIA A. S.; FONTANA, CARLA RAQUEL. Chlorin, Phthalocyanine, and Porphyrin Types Derivatives in Phototreatment of Cutaneous Manifestations: A Review. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 16, AUG 2 2019. (18/23015-7, 17/03552-5, 18/09088-1)

DOS SANTOS, DANILO M.; CHAGAS, PAULO A. M.; LEITE, ILAIALI S.; INADA, NATALIA M.; DE ANNUNZIO, SARAH R.; FONTANA, CARLA R.; CAMPANA-FILHO, SERGIO P.; CORREA, DANIEL S.. Core-sheath nanostructured chitosan-based nonwovens as a potential drug delivery system for periodontitis treatment. International Journal of Biological Macromolecules, v. 142, p. 521-534, JAN 1 2020. (17/20973-4, 18/23015-7, 18/09088-1, 13/07276-1, 17/12174-4)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; BASSO, FERNANDA GONCALVES; FONTANA, CARLA RAQUEL; APARECIDA GIRO, ELISA MARIA; BRIGHENTI, FERNANDA LOURENCAO. A systematic review and meta-analysis of the effect of photodynamic therapy for the treatment of oral mucositis. Photodiagnosis and Photodynamic Therapy, v. 34, JUN 2021. (18/18440-0, 20/07110-0, 21/01191-0, 19/08375-0, 18/23015-7)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; MARQUES, RAQUEL SOUZA; DE ANNUNZIO, SARAH RAQUEL; BRIGHENTI, FERNANDA LOURENCAO; FONTANA, CARLA RAQUEL. Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 14, JUL 2 2019. (18/23015-7, 18/18440-0, 18/09088-1)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FONTANA, CARLA RAQUEL; BASSO, FERNANDA GONCALVES; BRIGHETI, FERNANDA LOURENCAO. Photodynamic therapy for treating infected skin wounds: A systematic review and meta-analysis from randomized clinical trials. Photodiagnosis and Photodynamic Therapy, v. 40, p. 11-pg., 2022-12-01. (13/07276-1, 18/23015-7, 21/01191-0, 20/07110-0, 19/08375-0, 18/18440-0)