| Texto completo | |
| Autor(es): |
Fuentes-Leon, Fabiana
[1, 2]
;
de Oliveira, Andressa Peres
[1]
;
Quintero-Ruiz, Nathalia
[1]
;
Munford, Veridiana
[1]
;
Kajitani, Gustavo Satoru
[1]
;
Brum, Antonio Coimbra
[3]
;
Schuch, Andre Passaglia
[4]
;
Colepicolo, Pio
[5]
;
Sanchez-Lamar, Angel
[2]
;
Martins Menck, Carlos Frederico
[1]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, SP - Brazil
[2] Univ La Habana, Fac Biol, Dept Biol Vegetal, Havana - Cuba
[3] Univ Vale Rio dos Sinos, Sao Leopoldo, RS - Brazil
[4] Univ Fed Santa Maria, Ctr Ciencias Nat & Exatas, Dept Bioquim & Biol Mol, Santa Maria, RS - Brazil
[5] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, SP - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Photochemistry and Photobiology; v. 96, n. 6 JUL 2020. |
| Citações Web of Science: | 1 |
| Resumo | |
Sunlight ultraviolet (UV) radiation constitutes an important environmental genotoxic agent that organisms are exposed to, as it can damage DNA directly, generating pyrimidine dimers, and indirectly, generating oxidized bases and single-strand breaks (SSBs). These lesions can lead to mutations, triggering skin and eye disorders, including carcinogenesis and photoaging. Stratospheric ozone layer depletion, particularly in the Antarctic continent, predicts an uncertain scenario of UV incidence on the Earth in the next decades. This research evaluates the DNA damage caused by environmental exposure to late spring sunlight in the Antarctic Peninsula, where the ozone layer hole is more pronounced. These experiments were performed at the Brazilian Comandante Ferraz Antarctic Station, at King's George Island, South Shetlands Islands. For comparison, tropical regions were also analyzed. Samples of plasmid DNA were exposed to sunlight. Cyclobutane pyrimidine dimers (CPDs), oxidized base damage and SSBs were detected using specific enzymes. In addition, an immunological approach was used to detect CPDs. The results reveal high levels of DNA damage induced by exposure under the Antarctic sunlight, inversely correlated with ozone layer thickness, confirming the high impact of ozone layer depletion on the DNA damaging action of sunlight in Antarctica. (AU) | |
| Processo FAPESP: | 19/19435-3 - Papel de danos no DNA e função mitocondrial em envelhecimento vascular, imune e neurológico (DNA MoVINg) |
| Beneficiário: | Carlos Frederico Martins Menck |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |