Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related GenePIK3R1after Roux-en-Y Gastric Bypass

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Pinhel, Marcela A. S. ^{[1, 2]} ; Noronha, Natalia Y. ^[1] ; Nicoletti, Carolina F. ^[1] ; Pereira, Vanessa A. B. ^[1] ; de Oliveira, Bruno A. P. ^[1] ; Cortes-Oliveira, Cristiana ^[1] ; Salgado Jr, Wilson ; Barbosa, Fernando ^[3] ; Marchini, Julio S. ^[4] ; Souza, Doroteia R. S. ^[2] ; Nonino, Carla B. ^[4]

Afiliação do(s) autor(es)

^[1] Ribeirao Preto Med Sch, Hlth Sci Dept, Lab Nutrigen Studies, BR-14049900 Ribeirao Preto - Brazil

^[2] Sao Jose do Rio Preto Med Sch, Dept Mol Biol, BR-15090000 Sao Jose Do Rio Preto - Brazil

^[3] Sch Pharmaceut Sci Ribeirao Preto, BR-14040900 Ribeirao Preto - Brazil

^[4] Salgado Jr, Jr., Wilson, Ribeirao Preto Med Sch, Hlth Sci Dept, Lab Nutrigen Studies, BR-14049900 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 12 JUN 2020.
• Citações Web of Science: 0

Resumo

Weight regulation and the magnitude of weight loss after a Roux-en-Y gastric bypass (RYGB) can be genetically determined. DNA methylation patterns and the expression of some genes can be altered after weight loss interventions, including RYGB. The present study aimed to evaluate how the gene expression and DNA methylation ofPIK3R1, an obesity and insulin-related gene, change after RYGB. Blood samples were obtained from 13 women (35.9 +/- 9.2 years) with severe obesity before and six months after surgical procedure. Whole blood transcriptome and epigenomic patterns were assessed by microarray-based, genome-wide technologies. A total of 1966 differentially expressed genes were identified in the pre- and postoperative periods of RYGB. From these, we observed that genes involved in obesity and insulin pathways were upregulated after surgery. Then, thePIK3R1gene was selected for further RT-qPCR analysis and cytosine-guanine nucleotide (CpG) sites methylation evaluation. We observed that thePI3KR1gene was upregulated, and six DNA methylation CpG sites were differently methylated after bariatric surgery. In conclusion, we found that RYGB upregulates genes involved in obesity and insulin pathways. (AU)

• Processo FAPESP: 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/08784-4 - Reganho de peso no pós-operatório de cirurgia bariátrica: contribuições sociodemográficas, clínicas, nutricionais e genéticas
• Beneficiário: Carla Barbosa Nonino
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/12819-4 - Alterações genéticas, clínicas, bioquímicas e de variabilidade cardíaca na Obesidade grau III pré e pós-cirurgia bariátrica
• Beneficiário: Marcela Augusta de Souza Pinhel
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado