Changes in DNA Methylation and Gene Expression of Insulin and Obesity-Related GenePIK3R1after Roux-en-Y Gastric Bypass

Author(s):Show less -

Pinhel, Marcela A. S. ^{[1, 2]} ; Noronha, Natalia Y. ^[1] ; Nicoletti, Carolina F. ^[1] ; Pereira, Vanessa A. B. ^[1] ; de Oliveira, Bruno A. P. ^[1] ; Cortes-Oliveira, Cristiana ^[1] ; Salgado Jr, Wilson ; Barbosa, Fernando ^[3] ; Marchini, Julio S. ^[4] ; Souza, Doroteia R. S. ^[2] ; Nonino, Carla B. ^[4]

Affiliation

^[1] Ribeirao Preto Med Sch, Hlth Sci Dept, Lab Nutrigen Studies, BR-14049900 Ribeirao Preto - Brazil

^[2] Sao Jose do Rio Preto Med Sch, Dept Mol Biol, BR-15090000 Sao Jose Do Rio Preto - Brazil

^[3] Sch Pharmaceut Sci Ribeirao Preto, BR-14040900 Ribeirao Preto - Brazil

^[4] Salgado Jr, Jr., Wilson, Ribeirao Preto Med Sch, Hlth Sci Dept, Lab Nutrigen Studies, BR-14049900 Ribeirao Preto - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 12 JUN 2020.
• Web of Science Citations: 0

Abstract

Weight regulation and the magnitude of weight loss after a Roux-en-Y gastric bypass (RYGB) can be genetically determined. DNA methylation patterns and the expression of some genes can be altered after weight loss interventions, including RYGB. The present study aimed to evaluate how the gene expression and DNA methylation ofPIK3R1, an obesity and insulin-related gene, change after RYGB. Blood samples were obtained from 13 women (35.9 +/- 9.2 years) with severe obesity before and six months after surgical procedure. Whole blood transcriptome and epigenomic patterns were assessed by microarray-based, genome-wide technologies. A total of 1966 differentially expressed genes were identified in the pre- and postoperative periods of RYGB. From these, we observed that genes involved in obesity and insulin pathways were upregulated after surgery. Then, thePIK3R1gene was selected for further RT-qPCR analysis and cytosine-guanine nucleotide (CpG) sites methylation evaluation. We observed that thePI3KR1gene was upregulated, and six DNA methylation CpG sites were differently methylated after bariatric surgery. In conclusion, we found that RYGB upregulates genes involved in obesity and insulin pathways. (AU)

• FAPESP's process: 18/24069-3 - ReSEARCH: Recognizing Signatures of the Exposome to Anticipate the Risks for a Continuous Health
• Grantee: Fernando Barbosa Júnior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/08784-4 - Weight regain in the postoperative period of bariatric surgery: sociodemographic, clinical, nutritional and genetic contributions
• Grantee: Carla Barbosa Nonino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/12819-4 - Genetic, clinical, biochemical and heart rate variability changes in grade III Obesity pre and post bariatric surgery
• Grantee: Marcela Augusta de Souza Pinhel
• Support Opportunities: Scholarships in Brazil - Post-Doctoral