S-nitrosothiol-terminated Pluronic F127: Influence of microstructure on nitric oxide release

Autor(es)

Picheth, Guilherme F. ^[1] ; da Silva, Laura C. E. ^[1] ; Giglio, Leonardo P. ^[1] ; Plivelic, Tomas S. ^[2] ; de Oliveira, Marcelo G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, UNICAMP, POB 6154, BR-13083970 Campinas, SP - Brazil

^[2] Lund Univ, MAX IV Lab, POB 117, S-22100 Lund - Sweden

• Tipo de documento: Artigo Científico
• Fonte: Journal of Colloid and Interface Science; v. 576, p. 457-467, SEP 15 2020.
• Citações Web of Science: 0

Resumo

Hypothesis: Nitric oxide (NO)-releasing Pluronic F127 hydrogels (F127) containing dissolved S-nitrosothiols or pendant N-diazeniumdiolate (NONOate) groups have been described. The NO charging of these hydrogels is usually limited by their low stability or disruption of the micellar packing. S-nitrosothiol-terminated F127 may emerge as a new strategy for allowing NO delivery at different rates in biomedical applications. Experiments: Terminal hydroxyl groups of F127 were esterified and reduced to produce F127-mercaptopropionate (HS-F127-SH), which was subsequently S-nitrosated to generate S-nitrosothiolterminated F127 (ONS-F127-SNO). Micro-differential scanning calorimetry, H-1 NMR spin-spin relaxation (T-2), temperature-dependent small-angle X-ray scattering, and cryo-transmission electron microscopy, were used to determine the micellar packing structure, while real-time chemiluminescence NO detection and UV-Vis spectrophotometry were used to evaluate the kinetics of NO release. Findings: HS-F127-SH micellization and gelation processes were analogous to native F127, however, with a decreased short-range ordering of the micelles. ONS-F127-SNO hydrogels released NO thorough a preferentially intramicellar SNO dimerization reaction. Increasing ONS-F127-SNO concentration reduces the rate of SNO dimerization and increases the overall rate of NO release to the gas phase, opening up new possibilities for tailoring NO delivery from F127-based hydrogels. (C) 2020 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 18/14142-5 - Hidrogéis híbridos para liberação localizada e prolongada de óxido nítrico
• Beneficiário: Laura Caetano Escobar da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/04622-7 - Fibras eletrofiadas de PVA-GSNO: preparação e atividade biológica
• Beneficiário: Leonardo Pinheiro Giglio
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Iniciação Científica
• Processo FAPESP: 16/02414-5 - Biomateriais absorvíveis e tópicos para a liberação localizada de óxido nítrico
• Beneficiário: Marcelo Ganzarolli de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/19253-7 - Hidrogéis termosensíveis de Pluronic F127 funcionalizados com grupos terminais liberadores de óxido nítrico
• Beneficiário: Guilherme Fadel Picheth
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado