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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

S-nitrosothiol-terminated Pluronic F127: Influence of microstructure on nitric oxide release

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Picheth, Guilherme F. [1] ; da Silva, Laura C. E. [1] ; Giglio, Leonardo P. [1] ; Plivelic, Tomas S. [2] ; de Oliveira, Marcelo G. [1]
Total Authors: 5
[1] Univ Estadual Campinas, Inst Chem, UNICAMP, POB 6154, BR-13083970 Campinas, SP - Brazil
[2] Lund Univ, MAX IV Lab, POB 117, S-22100 Lund - Sweden
Total Affiliations: 2
Document type: Journal article
Source: Journal of Colloid and Interface Science; v. 576, p. 457-467, SEP 15 2020.
Web of Science Citations: 0

Hypothesis: Nitric oxide (NO)-releasing Pluronic F127 hydrogels (F127) containing dissolved S-nitrosothiols or pendant N-diazeniumdiolate (NONOate) groups have been described. The NO charging of these hydrogels is usually limited by their low stability or disruption of the micellar packing. S-nitrosothiol-terminated F127 may emerge as a new strategy for allowing NO delivery at different rates in biomedical applications. Experiments: Terminal hydroxyl groups of F127 were esterified and reduced to produce F127-mercaptopropionate (HS-F127-SH), which was subsequently S-nitrosated to generate S-nitrosothiolterminated F127 (ONS-F127-SNO). Micro-differential scanning calorimetry, H-1 NMR spin-spin relaxation (T-2), temperature-dependent small-angle X-ray scattering, and cryo-transmission electron microscopy, were used to determine the micellar packing structure, while real-time chemiluminescence NO detection and UV-Vis spectrophotometry were used to evaluate the kinetics of NO release. Findings: HS-F127-SH micellization and gelation processes were analogous to native F127, however, with a decreased short-range ordering of the micelles. ONS-F127-SNO hydrogels released NO thorough a preferentially intramicellar SNO dimerization reaction. Increasing ONS-F127-SNO concentration reduces the rate of SNO dimerization and increases the overall rate of NO release to the gas phase, opening up new possibilities for tailoring NO delivery from F127-based hydrogels. (C) 2020 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 18/14142-5 - Hybrid hydrogels for local and prolonged nitric oxide release
Grantee:Laura Caetano Escobar da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/04622-7 - Electrospun PVA-GSNO fibers: preparation and biological activity
Grantee:Leonardo Pinheiro Giglio
Support type: Scholarships abroad - Research Internship - Scientific Initiation
FAPESP's process: 16/02414-5 - Topical and absorbable biomaterials for the local nitric oxide release
Grantee:Marcelo Ganzarolli de Oliveira
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/19253-7 - Pluronic F127 thermosensitive hydrogels functionalized with terminal groups releasing nitric oxide
Grantee:Guilherme Fadel Picheth
Support type: Scholarships in Brazil - Post-Doctorate