Characterization of the Vaginal Microbiome in Women of Reproductive Age From 5 Regions in Brazil

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Marconi, Camila ^{[1, 2]} ; El-Zein, Mariam ^[3] ; Ravel, Jacques ^[4] ; Ma, Bing ^[4] ; Lima, Moises D. ^[5] ; Carvalho, Newton Sergio ^[6] ; Alves, Rosane R. F. ^[7] ; Parada, Cristina M. G. L. ^[8] ; Leite, Sandra H. M. ^[9] ; Giraldo, Paulo C. ^[10] ; Goncalves, Ana K. ^[11] ; Franco, Eduardo L. ^[3] ; Silva, Marcia G. ^[1]

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^[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Pathol, Botucatu, SP - Brazil

^[2] Fed Univ Parana UFPR, Dept Basic Pathol, Curitiba, Parana - Brazil

^[3] McGill Univ, Div Canc Epidemiol, Montreal, PQ - Canada

^[4] Univ Maryland, Sch Med, Inst Genom Sci, Baltimore, MD 21201 - USA

^[5] Fed Univ Paraiba UFPB, Dept Gynecol & Obstet, Joao Pessoa, Paraiba - Brazil

^[6] Fed Univ Parana UFPR, Dept Gynecol & Obstet, Curitiba, Parana - Brazil

^[7] Fed Univ Goias UFG, Dept Gynecol & Obstet, Goiania, Go - Brazil

^[8] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Nursing, Botucatu, SP - Brazil

^[9] State Univ Para UEPA, Dept Gynecol & Obstet, Belem, Para - Brazil

^[10] State Univ Campinas UNICAMP, Fac Med, Dept Gynecol & Obstet, Campinas, SP - Brazil

^[11] Fed Univ Rio Grande do Norte UFPR, Fac Med, Dept Gynecol & Obstet, Natal, RN - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SEXUALLY TRANSMITTED DISEASES; v. 47, n. 8, p. 562-569, AUG 2020.
• Citações Web of Science: 4

Resumo

Background Composition of the vaginal microbiome is strongly related to a woman's reproductive health and risk of sexually transmitted infections. Ethnoracial, behavioral, and environmental factors can influence microbiome. The Brazilian population is unique in terms of miscegenation of ethnic groups and behavioral characteristics across different regions. We aimed to characterize the vaginal microbiome of women from 5 geographical regions of Brazil. Methods We sequenced V3-V4 regions of 16S rRNA gene in vaginal samples of 609 reproductive-aged women. We performed logistic regression analyses to estimate odds ratios (OR) and 95% confidence intervals (CI) for the association between sociodemographic and behavioral factors withLactobacillus-depleted microbiome (community state type {[}CST] IV). Results Vaginal samples were grouped into 5 CST: CST I (L. crispatuspredominant, 30.5%), CST II (L. gasseripredominant, 4.4%), CST III (Lactobacillus inerspredominant, 36.5%), CST IV (Lactobacillus-depleted, 27.4%), and CST V (L. jenseniipredominant, 1.2%). Several factors were independently associated with CST IV, such as smoking (OR, 1.80; 95% CI, 1.02-3.18), number of partners (OR, 2.11; 95% CI, 1.20-3.70), and vaginal douching (OR, 2.24; 95% CI, 1.34-3.74). A protective effect was observed for milk/dairy intake (OR, 0.47; 95% CI, 0.27-0.82) and sitz bathing (OR, 0.43; 95% CI, 0.19-0.98). Conclusions Nearly two thirds of Brazilian women may be at an increased risk for adverse outcomes associated with a vaginal microbiota characterized by the depletion ofLactobacillusor dominance byL. iners,whose protective role has been widely questioned. Several factors related to sexual behavior and intimate hygiene were associated with CST IV. (AU)

• Processo FAPESP: 12/10403-2 - Caracterização do microbioma vaginal de mulheres brasileiras em idade reprodutiva
• Beneficiário: Camila Marconi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/22463-5 - Análise proteômica cérvico-vaginal na vaginose bacteriana: associação com a composição bacteriana e resposta ao tratamento com metronidazol
• Beneficiário: Camila Marconi
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 12/16800-3 - Caracterização do microbioma vaginal de mulheres brasileiras em idade reprodutiva
• Beneficiário: Márcia Guimarães da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular