Exploring the impact of morphology, multiple sequence alignment and choice of optimality criteria in phylogenetic inference: a case study with the Neotropical orb-weaving spider genus Wagneriana (Araneae: Araneidae)

Autor(es)

Cabra-Garcia, Jimmy ^{[1, 2]} ; Hormiga, Gustavo ^[3]

Afiliação do(s) autor(es)

^[1] Univ Valle, Dept Biol, Cali 25360 - Colombia

^[2] Univ Sao Paulo, Dept Zool, Inst Biociencias, BR-05508090 Sao Paulo, SP - Brazil

^[3] George Washington Univ, Dept Biol Sci, Washington, DC 20052 - USA

• Tipo de documento: Artigo Científico
• Fonte: ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY; v. 188, n. 4, p. 976-1151, APR 2020.
• Citações Web of Science: 1

Resumo

We present a total evidence phylogenetic analysis of the Neotropical orb-weaving spider genus Wagneriana and discuss the phylogenetic impacts of methodological choices. We analysed 167 phenotypic characters and nine loci scored for 115 Wagneriana and outgroups, including 46 newly sequenced species. We compared total evidence analyses and molecular-only analyses to evaluate the impact of phenotypic evidence, and we performed analyses using the programs POY, TNT, RAxML, GARLI, IQ-TREE and MrBayes to evaluate the effects of multiple sequence alignment and optimality criteria. In all analyses, Wagneriana carimagua and Wagneriana uropygialis were nested in the genera Parawixia and Alpaida, respectively, and the remaining species of Wagneriana fell into three main clades, none of which formed a pair of sister taxa. However, sister-group relationships among the main clades and their internal relationships were strongly influenced by methodological choices. Alignment methods had comparable topological effects to those of optimality criteria in terms of `subtree pruning and regrafting' moves. The inclusion of phenotypic evidence, 2.80-3.05% of the total evidence matrices, increased support irrespective of the optimality criterion used. The monophyly of some groups was recovered only after the addition of morphological characters. A new araneid genus, Popperaneus gen. nov., is erected, and Paraverrucosa is resurrected. Four new synonymies and seven new combinations are proposed. (AU)

• Processo FAPESP: 13/20262-0 - Sistemática e evolução das aranhas orbitelas do gênero Wagneriana Cambridge, 1904 (Araneae: Araneidae)
• Beneficiário: Jimmy Jair Cabra Garcia
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/04437-2 - Explorando o sinal filogenético da morfologia: um estudo de caso no gênero de aranhas orbitelas Wagneriana F.O.P.-Cambridge, 1904 (Araneae: Araneidae)
• Beneficiário: Jimmy Jair Cabra Garcia
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado