A Highly Glucose Tolerant ss-Glucosidase from Malbranchea pulchella (MpBg3) Enables Cellulose Saccharification

Autor(es)

Oliveira Monteiro, Lummy Maria ^[1] ; Vici, Ana Claudia ^[2] ; Pinheiro, Matheus Pinto ^[3] ; Heinen, Paulo Ricardo ^[1] ; Cavalcante de Oliveira, Arthur Henrique ^[2] ; Ward, Richard John ^[2] ; Prade, Rolf Alexander ^[4] ; Buckeridge, Marcos S. ^[5] ; Teixeira de Moraes Polizeli, Maria de Lourdes ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Bandeirantes Av 3-900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Bandeirantes Av 3-900, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Ctr Nacl Pesquisa Energia & Mat CNPEM, Lab Nacl Biociencia LNBio, Campinas, SP - Brazil

^[4] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 - USA

^[5] Univ Sao Paulo, Inst Biociencias, Matao St 277, BR-05508090 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 APR 24 2020.
• Citações Web of Science: 0

Resumo

beta -glucosidases catalyze the hydrolysis beta -1,4, beta -1,3 and beta -1,6 glucosidic linkages from non-reducing end of short chain oligosaccharides, alkyl and aryl beta -D-glucosides and disaccharides. They catalyze the rate-limiting reaction in the conversion of cellobiose to glucose in the saccharification of cellulose for second-generation ethanol production, and due to this important role the search for glucose tolerant enzymes is of biochemical and biotechnological importance. In this study we characterize a family 3 glycosyl hydrolase (GH3) beta -glucosidase (Bgl) produced by Malbranchea pulchella (MpBgl3) grown on cellobiose as the sole carbon source. Kinetic characterization revealed that the MpBgl3 was highly tolerant to glucose, which is in contrast to many Bgls that are completely inhibited by glucose. A 3D model of MpBgl3 was generated by molecular modeling and used for the evaluation of structural differences with a Bgl3 that is inhibited by glucose. Taken together, our results provide new clues to understand the glucose tolerance in GH3 beta -glucosidases. (AU)

• Processo FAPESP: 10/52322-3 - Bioprospecção de fungos filamentosos produtores de holoenzimas com aplicação em biorefinaria
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Temático