Utilization of sugarcane straw for production of beta-glucan biopolymer by Lasiodiplodia theobromae CCT 3966 in batch fermentation process

Autor(es)

Abdeshahian, Peyman ^[1] ; Ascencio, Jesus Jimenez ^[1] ; Philippini, Rafael R. ^[1] ; Fernandes Antunes, Felipe Antonio ^[1] ; dos Santos, Julio Cesar ^[1] ; da Silva, Silvio Silverio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Engn Sch Lorena, Dept Biotechnol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 314, OCT 2020.
• Citações Web of Science: 0

Resumo

beta-Glucans as emerging biopolymer are widely produced by microorganisms in fermentation processes using commercial sugars which make process non-economic. Lignocellulosic substances are inexpensive carbon sources, which could be exploited for sustainable production of beta-glucans. In this study, a lignocellulosic material, namely sugarcane straw (SCS) was utilized for the production of extracellular beta-glucan by Lasiodiplodia theobromae CCT3966. SCS was subjected to acid and subsequent alkaline pretreatment, followed by enzymatic saccharification using cellulase enzyme. Quantity of 48.65 g/L glucose was released after enzymatic hydrolysis. beta-Glucan production was performed by cultivation of fungal strain in SCS hydrolysate at 28 degrees C and initial culture pH 7. Highest beta-glucan yield and productivity of 0.047 gg(-1) and 0.014 gL(-1) h(-1), respectively was obtained at 72 h fermentation time. Kinetic study of beta-glucan production revealed experimental biosynthesis of beta-glucan from SCS hydrolysate followed the trend generated by Logistic and Luedeking-Piret models. Chemical structure of biopolymer produced showed beta-glucan constitution. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 18/14095-7 - Novas estratégias integradas para produção econômica de beta-glucana a partir de resíduos agroindustriais e roteiro para comercialização no Brasil
• Beneficiário: Peyman Abdeshahian
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado