Biocatalytic production of isoamyl acetate from fusel oil in supercritical CO2

Autor(es)

Baiao Dias, Arthur Luiz ^[1] ; Hatami, Tahmasb ^[1] ; Martinez, Julian ^[1] ; Ciftci, Ozan N. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Coll Food Engn, Food Engn Dept, UNICAMP, BR-13083862 Campinas, SP - Brazil

^[2] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68588 - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SUPERCRITICAL FLUIDS; v. 164, OCT 1 2020.
• Citações Web of Science: 0

Resumo

Isoamyl acetate esters were enzymatically synthesized from fusel oil, a by-product generated in bioethanol industrial plants, in supercritical carbon dioxide (SC-CO2) using immobilized lipase (Lipozyme 435). Effects of enzyme load (2.5-10.0 %), substrates' molar ratio (acetic anhydride to fusel oil; 1:1-4:1), acyl donor (acetic anhydride, ethyl acetate, and acetic acid) and solvent type (SC-CO2, n-hexane, and solvent-free system) on the biocatalytic isoamyl acetate conversion and the specific productivity were investigated. Higher volumetric expansions of the reaction medium were observed at higher temperature (60 degrees C) between 10 and 20 MPa. A monophasic system was observed at 15 MPa and 60 degrees C. Acetic anhydride and SC-CO2 were the best acyl donor and solvent, respectively. An elementary mathematical model was adjusted to the experimental data. 15 MPa, 60 degrees C, 2:1 substrates' molar ratio and 2.5 % of enzyme concentration yielded the highest conversion (88.4 +/- 0.5 %) and specific productivity (36.9 +/- 0.2 %). (C) 2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 17/23670-2 - Biorrefinaria para aproveitamento de subprodutos do processamento de maracujá com tecnologias de alta pressão
• Beneficiário: Julian Martínez
• Modalidade de apoio: Auxílio à Pesquisa - Regular