Sugarcane cystatin CaneCPI-1 promotes osteogenic differentiation in human dental pulp cells: a new insight into cysteine proteases inhibitors

Autor(es)

Rodrigues, E. M. ^[1] ; Viola, K. S. ^[1] ; Gomes-Cornelio, A. L. ^[1] ; Soares-Costa, A. ^[2] ; Henrique-Silva, F. ^[2] ; Rossa-Junior, C. ^[3] ; Guerreiro-Tanomaru, J. M. ^[1] ; Tanomaru-Filho, M. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Restorat Dent, Dent Sch, Araraquara, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos - Brazil

^[3] Dent Sch Sao Paulo State Univ, Dept Diag & Surg, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; AUG 2020.
• Citações Web of Science: 0

Resumo

Aim To investigate the biocompatibility, type of cell death, osteogenic bioactivity and mRNA expression of the osteogenic markers, induced by CaneCPI-1 in human dental pulp cells (hDPCs). Methodology hDPCs exposed to CaneCPI-1 and not exposed (control) were evaluated for cell viability by the 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay; apoptosis by flow cytometry; alkaline phosphatase (ALP) activity by calculation of thymolphthalein release; gene expression of bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX2), ALP, osteocalcin (OC), bone sialoprotein (BSP) by qPCR; and mineralized nodules production by using alizarin red staining. The data were analysed by one-way analysis of variance (anova) and Turkey's post-test, two-wayanovaand Bonferroni post-test or t-test (P < 0.05). Results CaneCPI-1 induced no apoptosis and had no cytotoxic effect, except in the concentration of 33.20 mu m, in which cell viability was significantly lower than the control (alpha-MEM nonosteogenic medium serum-free) (P < 0.05). There was significantly greater ALP activity, greater expression of the BMP-2, RUNX2, ALP, OC and BSP genes and greater mineralized nodules production in the CaneCPI-1 group in comparison with the control or osteogenic alpha-MEM control (alpha-MEM osteogenic medium - L-ascorbic acid and beta-glycerophosphate) (P < 0.05). Conclusions CaneCPI-1 was cytocompatible and also induced the differentiation of hDPCs in osteogenic phenotypein vitro. CaneCPI-1 is a promising molecule to induce pulp repair. (AU)

• Processo FAPESP: 17/19049-0 - Avaliação das etapas do tratamento de canais radiculares, e das propriedades físico-químicas e biológicas de materiais à base de silicato tricálcico
• Beneficiário: Mario Tanomaru Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular