| Full text | |
| Author(s): |
Rodrigues, E. M.
[1]
;
Viola, K. S.
[1]
;
Gomes-Cornelio, A. L.
[1]
;
Soares-Costa, A.
[2]
;
Henrique-Silva, F.
[2]
;
Rossa-Junior, C.
[3]
;
Guerreiro-Tanomaru, J. M.
[1]
;
Tanomaru-Filho, M.
[1]
Total Authors: 8
|
| Affiliation: | [1] Sao Paulo State Univ, Dept Restorat Dent, Dent Sch, Araraquara, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos - Brazil
[3] Dent Sch Sao Paulo State Univ, Dept Diag & Surg, Araraquara, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | International Endodontic Journal; AUG 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Aim To investigate the biocompatibility, type of cell death, osteogenic bioactivity and mRNA expression of the osteogenic markers, induced by CaneCPI-1 in human dental pulp cells (hDPCs). Methodology hDPCs exposed to CaneCPI-1 and not exposed (control) were evaluated for cell viability by the 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay; apoptosis by flow cytometry; alkaline phosphatase (ALP) activity by calculation of thymolphthalein release; gene expression of bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX2), ALP, osteocalcin (OC), bone sialoprotein (BSP) by qPCR; and mineralized nodules production by using alizarin red staining. The data were analysed by one-way analysis of variance (anova) and Turkey's post-test, two-wayanovaand Bonferroni post-test or t-test (P < 0.05). Results CaneCPI-1 induced no apoptosis and had no cytotoxic effect, except in the concentration of 33.20 mu m, in which cell viability was significantly lower than the control (alpha-MEM nonosteogenic medium serum-free) (P < 0.05). There was significantly greater ALP activity, greater expression of the BMP-2, RUNX2, ALP, OC and BSP genes and greater mineralized nodules production in the CaneCPI-1 group in comparison with the control or osteogenic alpha-MEM control (alpha-MEM osteogenic medium - L-ascorbic acid and beta-glycerophosphate) (P < 0.05). Conclusions CaneCPI-1 was cytocompatible and also induced the differentiation of hDPCs in osteogenic phenotypein vitro. CaneCPI-1 is a promising molecule to induce pulp repair. (AU) | |
| FAPESP's process: | 17/19049-0 - Evaluation of the root canal treatment procedures, physicochemical and biological properties of tricalcium silicate-based materials |
| Grantee: | Mario Tanomaru Filho |
| Support type: | Regular Research Grants |