Efficient photodynamic inactivation ofCandida albicansby porphyrin and potassium iodide co-encapsulation in micelles

Autor(es):Mostrar menos -

Castro, Kelly A. D. F. ^[1] ; Brancini, Guilherme T. P. ^[2] ; Costa, Leticia D. ^[3] ; Biazzotto, Juliana C. ^[1] ; Faustino, M. Amparo F. ^[3] ; Tome, Augusto C. ^[3] ; Neves, M. Graca P. M. S. ^[3] ; Almeida, Adelaide ^[4] ; Hamblin, Michael R. ^{[5, 6]} ; da Silva, Roberto S. ^[1] ; Braga, Gilberto U. L. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto - Brazil

^[3] Univ Aveiro, Dept Chem, LAQV REQUIMTE, P-3810193 Aveiro - Portugal

^[4] Univ Aveiro, Dept Biol, CESAM, P-3810193 Aveiro - Portugal

^[5] Harvard Med Sch, Wellman Ctr Photomed, Massachusetts Gen Hosp, Boston, MA 02114 - USA

^[6] Univ Johannesburg, Fac Hlth Sci, Laser Res Ctr, ZA-2028 Doornfontein - South Africa

• Tipo de documento: Artigo Científico
• Fonte: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 19, n. 8, p. 1063-1071, AUG 1 2020.
• Citações Web of Science: 2

Resumo

Photodynamic inactivation of bacterial and fungal pathogens is a promising alternative to the extensive use of conventional single-target antibiotics and antifungal agents. The combination of photosensitizers and adjuvants can improve the photodynamic inactivation efficiency. In this regard, it has been shown that the use of potassium iodide (KI) as adjuvant increases pathogen killing. Following our interest in this topic, we performed the co-encapsulation of a neutral porphyrin photosensitizer (designated asP1) and KI into micelles and tested the obtained nanoformulations against the human pathogenic fungusCandida albicans. The results of this study showed that the micelles containingP1and KI displayed a better photodynamic performance towardsC. albicansthanP1and KI in solution. It is noteworthy that higher concentrations of KI within the micelles resulted in increased killing ofC. albicans. Subcellular localization studies by confocal fluorescence microscopy revealed thatP1was localized in the cell cytoplasm, but not in the nuclei or mitochondria. Overall, our results show that a nanoformulation containing a photosensitizer plus an adjuvant is a promising approach for increasing the efficiency of photodynamic treatment. Actually, the use of this strategy allows a considerable decrease in the amount of both photosensitizer and adjuvant required to achieve pathogen killing. (AU)

• Processo FAPESP: 16/12707-0 - Citotoxicidade e foto-citotoxicidade de compostos rutênio-ftalocianinas como produtores de óxido nítrico e oxigênio singleto em linhagens de células cancerígenas. Proposta para uma nova classe de metalo-drogas
• Beneficiário: Roberto Santana da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/11386-5 - Estudo mecanístico da percepção e da resposta a luz visível no fungo entomopatogênico Metarhizium acridum
• Beneficiário: Gilberto Úbida Leite Braga
• Modalidade de apoio: Auxílio à Pesquisa - Regular