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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Efficient photodynamic inactivation ofCandida albicansby porphyrin and potassium iodide co-encapsulation in micelles

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Author(s):
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Castro, Kelly A. D. F. [1] ; Brancini, Guilherme T. P. [2] ; Costa, Leticia D. [3] ; Biazzotto, Juliana C. [1] ; Faustino, M. Amparo F. [3] ; Tome, Augusto C. [3] ; Neves, M. Graca P. M. S. [3] ; Almeida, Adelaide [4] ; Hamblin, Michael R. [5, 6] ; da Silva, Roberto S. [1] ; Braga, Gilberto U. L. [2]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto - Brazil
[3] Univ Aveiro, Dept Chem, LAQV REQUIMTE, P-3810193 Aveiro - Portugal
[4] Univ Aveiro, Dept Biol, CESAM, P-3810193 Aveiro - Portugal
[5] Harvard Med Sch, Wellman Ctr Photomed, Massachusetts Gen Hosp, Boston, MA 02114 - USA
[6] Univ Johannesburg, Fac Hlth Sci, Laser Res Ctr, ZA-2028 Doornfontein - South Africa
Total Affiliations: 6
Document type: Journal article
Source: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 19, n. 8, p. 1063-1071, AUG 1 2020.
Web of Science Citations: 2
Abstract

Photodynamic inactivation of bacterial and fungal pathogens is a promising alternative to the extensive use of conventional single-target antibiotics and antifungal agents. The combination of photosensitizers and adjuvants can improve the photodynamic inactivation efficiency. In this regard, it has been shown that the use of potassium iodide (KI) as adjuvant increases pathogen killing. Following our interest in this topic, we performed the co-encapsulation of a neutral porphyrin photosensitizer (designated asP1) and KI into micelles and tested the obtained nanoformulations against the human pathogenic fungusCandida albicans. The results of this study showed that the micelles containingP1and KI displayed a better photodynamic performance towardsC. albicansthanP1and KI in solution. It is noteworthy that higher concentrations of KI within the micelles resulted in increased killing ofC. albicans. Subcellular localization studies by confocal fluorescence microscopy revealed thatP1was localized in the cell cytoplasm, but not in the nuclei or mitochondria. Overall, our results show that a nanoformulation containing a photosensitizer plus an adjuvant is a promising approach for increasing the efficiency of photodynamic treatment. Actually, the use of this strategy allows a considerable decrease in the amount of both photosensitizer and adjuvant required to achieve pathogen killing. (AU)

FAPESP's process: 16/12707-0 - Cytotoxicity and photo-cytotoxicity of new ruthenium-phthalocyanines compounds as nitric oxide and oxygen singlet producers in cancer cell lines. an innovative purpose for metal based drug
Grantee:Roberto Santana da Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 16/11386-5 - Mechanistic study of perception and response to visible light in the entomopathogenic fungus Metarhizium acridum
Grantee:Gilberto Úbida Leite Braga
Support Opportunities: Regular Research Grants