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Photodinamic inactivation using curcumin conjugated to pluronic ®F-127 on Streptococcus mutans and Candida albicans biofilm

Grant number: 17/17839-4
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): December 01, 2017
Effective date (End): March 31, 2019
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Alessandra Nara de Souza Rastelli
Grantee:Diego Dantas Lopes dos Santos
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated research grant:13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID


The photodynamic therapy is described as a promising treatment to inactivate oral microorganisms. Therefore, curcumin is a hydrophobic photosensitizer that can compromise the efficiency, the development of studies that investigate the effect of curcumin when associated with polymeric micelles are important. Thus, the purpose of this study is to evaluate the efficacy of photodynamic inactivation (PDI) on a Streptococcus mutans and Candida albicans biofilm using curcumin conjugated or not with micelles as photosensitizer, irradiated by a LED light source. The fluorescence and optical absorption spectroscopies, the minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), and minimal fungicidal concentration (MFC) will be determined. Single species biofilms will be induced using a 96-well plate followed by duo species biofilms. Colony forming unit (UFC/mL) will be obtained after determination of MIC, MBC and MFC. The interaction and cellular viability of the biofilms will be analyzed by confocal microscopy using BacLight® LIVE/DEAD dye. Different Groups will be evaluated: PSM+L+ (Photosensitizer + micelles + light), PSD+L+ (Photosensitizer + DMSO + light), PS-L- (negative control), PS-L+ (Light group), chlorhexidine 2% (positive control) and nistatine (positive control). After evaluation of normality and homoscedasticity, if they are met, the data will be submitted to analysis of Variance (ANOVA) and multiple comparison, otherwise, non-parametric analysis will be used. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DANTAS LOPES DOS SANTOS, DIEGO; BESEGATO, JOAO FELIPE; DEMELO, PRISCILA BORGES GOBBO; OSHIRO JUNIOR, JOAO AUGUSTO; CHORILLI, MARLUS; DENG, DONGMEI; BAGNATO, VANDERLEI SALVADOR; RASTELLI, ALESSANDRA NARA DE SOUZA. Curcumin-loaded Pluronic (R) F-127 Micelles as a Drug Delivery System for Curcumin-mediated Photodynamic Therapy for Oral Application. Photochemistry and Photobiology, v. 97, n. 5, p. 1072-1088, . (13/07276-1, 17/17839-4)
LOPES DOS SANTOS, DIEGO DANTAS; BESEGATO, JOAO FELIPE; GOBBO DE MELO, PRISCILA BORGES; OSHIRO JUNIOR, JOAO AUGUSTO; CHORILLI, MARLUS; DENG, DONGMEI; BAGNATO, VANDERLEI SALVADOR; DE SOUZA RASTELLI, ALESSANDRA NARA. Effect of curcumin-encapsulated Pluronic(R) F-127 over duo-species biofilm of Streptococcus mutans and Candida albicans. Lasers in Medical Science, . (13/07276-1, 17/17839-4)
DE SOUZA RASTELLI, ALESSANDRA NARA; DIAS, HERCULES BEZERRA; CARRERA, EMANUELLE TEIXEIRA; PEDROSO DE BARROS, ANA CLAUDIA; LOPES DOS SANTOS, DIEGO DANTAS; PANHOCA, VITOR HUGO; BAGNATO, VANDERLEI SALVADOR. Violet LED with low concentration carbamide peroxide for dental bleaching: A case report. Photodiagnosis and Photodynamic Therapy, v. 23, p. 270-272, . (17/17839-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SANTOS, Diego Dantas Lopes dos. Photodynamic inactivation using Pluronic® F-127 conjugated curcumin on the biofilm of Streptococcus mutans. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.

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