Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis

Autor(es):Mostrar menos -

Mompeon, Ana ^{[1, 2]} ; Ortega-Paz, Luis ^[3] ; Vidal-Gomez, Xavier ^{[1, 2]} ; Januario Costa, Tiago ^[4] ; Perez-Cremades, Daniel ^{[1, 2]} ; Garcia-Blas, Sergio ^[5] ; Brugaletta, Salvatore ^{[4, 3]} ; Sanchis, Juan ^[5] ; Sabate, Manel ^{[4, 3]} ; Novella, Susana ^{[1, 2]} ; Paula Dantas, Ana ^{[4, 3]} ; Hermenegildo, Carlos ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Valencia, Fac Med & Dent, Dept Physiol, Valencia - Spain

^[2] INCLIVA Biomed Res Inst, Valencia - Spain

^[3] Hosp Clin & Prov Barcelona HCPB, Inst Clin Cardiovasc ICCV, Barcelona - Spain

^[4] Inst Invest Biomed August Pi & Sunyer IDIBAPS, Barcelona - Spain

^[5] Univ Valencia, Ctr Invest Biomed Red Enfermedades Cardiovasc CIB, INCLIVA Biomed Res Inst, Cardiol Div, Hosp Clin Univ Valencia HCUV, Valencia - Spain

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 MAR 25 2020.
• Citações Web of Science: 0

Resumo

Despite the promising value of miRNAs in the diagnostic and prognostic of cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances in studies may interfere with miRNA profile and affect their results. This study determines if the blood starting material is a source of variance in miRNA profile by performing a paired comparison in plasma and serum of the expression of primary miRNAs associated with CVD. Circulating miRNA yield was similar in both plasma and serum, although a significant increase was observed in patients with Non-ST-elevation myocardial infarction (NSTEMI) compared to control volunteers. When normalized by the expression of miR-484, different patterns of miRNA expression between serum and plasma. Although NSTEMI modified the expression of miR-1 and miR-208 in both serum and plasma, plasma displayed a higher variance than serum (Levene's test p<0.01). For miR-133a and miR-26a, differences were only detected in serum (p=0.0240), and conversely, miR-499a showed differences only in plasma of NSTEMI (p=0.001). Interestingly, miR-21 showed an opposite pattern of expression, being increased in serum (2(-<Delta>Delta Ct): 5.7, p=0.0221) and decreased in plasma (2(-Delta Delta Ct): 0.5, p=0.0107). Plasma and serum exhibit different patterns of circulating miRNA expression in NSTEMI and suggest that results from studies with different starting material could not be comparable. (AU)

• Processo FAPESP: 15/26690-9 - Análise da regulação transcricional e pós-transcricional dos receptores de estrógeno (ER) nas alterações em carótidas mediadas por estrógeno no modelo murino fêmea de envelhecimento.
• Beneficiário: Tiago Januário da Costa
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado