| Texto completo | |
| Autor(es): |
Okuda, Ellen K.
[1]
;
Gonzales-Zubiate, Fernando A.
[1, 2]
;
Gadal, Olivier
[3]
;
Oliveira, Carla C.
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil
[2] Yachay Tech Univ, Sch Biol Sci & Engn, San Miguel De Urcuqui - Ecuador
[3] Univ Toulouse, Ctr Biol Integrat CBI, Lab Biol Mol Eucaryote, UPS, CNRS, Toulouse - France
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Biological Chemistry; v. 295, n. 32, p. 11195-11213, AUG 7 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
The RNA exosome is a multisubunit protein complex involved in RNA surveillance of all classes of RNA, and is essential for pre-rRNA processing. The exosome is conserved throughout evolution, present in archaea and eukaryotes from yeast to humans, where it localizes to the nucleus and cytoplasm. The catalytically active subunit Rrp44/Dis3 of the exosome in budding yeast (Saccharomyces cerevisiae) is considered a protein present in these two subcellular compartments, and here we report that it not only localizes mainly to the nucleus, but is concentrated in the nucleolus, where the early pre-rRNA processing reactions take place. Moreover, we show by confocal microscopy analysis that the core exosome subunits Rrp41 and Rrp43 also localize largely to the nucleus and strongly accumulate in the nucleolus. These results shown here shed additional light on the localization of the yeast exosome and have implications regarding the main function of this RNase complex, which seems to be primarily in early pre-rRNA processing and surveillance. (AU) | |
| Processo FAPESP: | 17/17777-9 - Estudo dos mecanismos de transporte celular de subunidades do complexo exossomo em Saccharomyces cerevisiae |
| Beneficiário: | Ellen Kazumi Okuda |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 15/06477-9 - Estudo funcional de componentes do exossomo e do spliceossomo de Saccharomyces cerevisiae e de seu papel no controle pós-transcricional de expressão gênica |
| Beneficiário: | Carla Columbano de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/19451-6 - Estudo dos mecanismos de transporte celular da subunidade Rrp44 do exossomo em Saccharomyces cerevisiae |
| Beneficiário: | Ellen Kazumi Okuda |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Mestrado |