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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nucleolar localization of the yeast RNA exosome subunit Rrp44 hints at early pre-rRNA processing as its main function

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Author(s):
Okuda, Ellen K. [1] ; Gonzales-Zubiate, Fernando A. [1, 2] ; Gadal, Olivier [3] ; Oliveira, Carla C. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil
[2] Yachay Tech Univ, Sch Biol Sci & Engn, San Miguel De Urcuqui - Ecuador
[3] Univ Toulouse, Ctr Biol Integrat CBI, Lab Biol Mol Eucaryote, UPS, CNRS, Toulouse - France
Total Affiliations: 3
Document type: Journal article
Source: Journal of Biological Chemistry; v. 295, n. 32, p. 11195-11213, AUG 7 2020.
Web of Science Citations: 0
Abstract

The RNA exosome is a multisubunit protein complex involved in RNA surveillance of all classes of RNA, and is essential for pre-rRNA processing. The exosome is conserved throughout evolution, present in archaea and eukaryotes from yeast to humans, where it localizes to the nucleus and cytoplasm. The catalytically active subunit Rrp44/Dis3 of the exosome in budding yeast (Saccharomyces cerevisiae) is considered a protein present in these two subcellular compartments, and here we report that it not only localizes mainly to the nucleus, but is concentrated in the nucleolus, where the early pre-rRNA processing reactions take place. Moreover, we show by confocal microscopy analysis that the core exosome subunits Rrp41 and Rrp43 also localize largely to the nucleus and strongly accumulate in the nucleolus. These results shown here shed additional light on the localization of the yeast exosome and have implications regarding the main function of this RNase complex, which seems to be primarily in early pre-rRNA processing and surveillance. (AU)

FAPESP's process: 17/17777-9 - Study of the celular transport mechanism of the exosome's subunits in Saccharomyces cerevisiae
Grantee:Ellen Kazumi Okuda
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 15/06477-9 - Functional study of the components of the Saccharomyces cerevisiae exosome and the spliceosome, and their role in posttranscriptional control of gene expression
Grantee:Carla Columbano de Oliveira
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/19451-6 - Study of the cellular transport mechanism of the RNA exosome subunit Rrp44 in Saccharomyces cerevisiae
Grantee:Ellen Kazumi Okuda
Support Opportunities: Scholarships abroad - Research Internship - Master's degree