Bioprospecting of probiotics with antimicrobial activities against Salmonella Heidelberg and that produce B-complex vitamins as potential supplements in poultry nutrition

Autor(es)

Sabo, Sabrina da Silva ^[1] ; Mendes, Maria Anita ^[2] ; Araujo, Elias da Silva ^[3] ; de Almeida Muradian, Ligia Bicudo ^[3] ; Makiyama, Edson Naoto ^[4] ; Guy LeBlanc, Jean ^[5] ; Borelli, Primavera ^[4] ; Fock, Ricardo Ambrosio ^[4] ; Knobl, Terezinha ^{[6, 7]} ; de Souza Oliveira, Ricardo Pinheiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Chem Engn Dept, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Food & Expt Nutr, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

^[5] CERELA CONICET, San Miguel De Tucuman - Argentina

^[6] Univ Sao Paulo, Sao Paulo - Brazil

^[7] Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 APR 29 2020.
• Citações Web of Science: 0

Resumo

The demand for animal protein for human consumption has been risen exponentially. Modern animal production practices are associated with the regular use of antibiotics, potentially increasing the emerging multi-resistant bacteria, which may have a negative impact on public health. In poultry production, substances capable of maximizing the animals' performance and displaying an antimicrobial activity against pathogens are very well desirable features. Probiotic can be an efficient solution for such a task. In the present work, lactic acid bacteria (LAB) were isolated from chicken cecum and screened for their antagonistic effect towards many pathogens. Their capacity of producing the B-complex vitamins folate and riboflavin were also evaluated. From 314 isolates, three (C43, C175 and C195) produced Bacteriocin-Like Inhibitory Substances (BLIS) against Staphylococcus aureus (inhibition zones of 18.9, 21.5, 19.5mm, respectively) and also inhibited the growth of Salmonella Heidelberg. The isolate C43 was identified as Enterococcus faecium, while C173 and C195 were both identified as Lactococcus lactis subsp. lactis. Moreover, the isolates L. lactis subsp. lactis strains C173 and C195 demonstrated high potential to be used as probiotic in poultry feed, in addition to their advantage of producing folate (58.0 and 595.5ng/mL, respectively) and riboflavin (223.3 and 175.0ng/mL, respectively). (AU)

• Processo FAPESP: 18/25511-1 - Bioprospecção de bactérias probióticas bacteriocinogênicas: da otimização do cultivo à aplicação em sistemas de produção animal
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/16494-3 - Bioprospecção de bactérias probióticas produtoras de bacteriocinas e vitaminas: uma aplicação como aditivo alimentar em aves para consumo humano
• Beneficiário: Sabrina da Silva Sabo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado