Microbiological analysis of endodontically treated teeth with apical periodontitis before and after endodontic retreatment

Autor(es)

Barbosa-Ribeiro, Marlos ^{[1, 2]} ; Arruda-Vasconcelos, Rodrigo ^[1] ; Louzada, Lidiane M. ^[1] ; dos Santos, Danielle G. ^[3] ; Andreote, Fernando D. ^[3] ; Gomes, Brenda P. F. A. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Restorat Dent, Div Endodont, Av Limeira 901, Piracicaba, SP - Brazil

^[2] Univ Ctr UniRuy Wyden, Dept Endodont Sch Dent, Salvador, BA - Brazil

^[3] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Soil Sci, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 4 AUG 2020.
• Citações Web of Science: 1

Resumo

Objective To characterize the microbiota of teeth with endodontic treatment failure by 16S ribosomal RNA genetic sequencing (GS) and PCR at the different phases of the endodontic retreatment and to associate the presence of specific bacteria with clinical and radiographic features in teeth with apical periodontitis. Materials and methods Twenty infected root canals of single-rooted teeth were selected. Samples were collected with sterile paper points before chemo-mechanical preparation (CMP) (S1), after CMP (S2) and after 30 days of intracanal medication (ICM) (S3). Microbial identification was performed using GS and PCR. Tukey-Kramer post hoc test and post hoc ANOVA were used for intergroup analysis. Pairedttest and repeated-measures ANOVA were applied for intragroup analysis, at a significance level of 5%. Results A total of 89 strains were identified using GS. Sixty-five strains were recovered in S1 and 15 strains in S2, and 9 strains remained in S3.Enterococcus faecaliswas the most predominant bacteria. Gram-positive cocci bacteria predominated. Gram-negative species were also detected. Using species-specific PCR primers to detect seven species, the most prevalent ones at all the phases of the endodontic retreatment wereE. faecalisandPorphyromonas gingivalis. However,Parvimonas micraandP. gingivaliswere associated with previous pain,P. gingivaliswas associated with tenderness to percussion andE. faecalis,Fusobacterium nucleatumandP. gingivaliswere associated with periapical lesion > 3 mm. Conclusions In conclusion, the microbiota of persistent infection is polymicrobial with predominance ofE. faecalisandP. gingivalisin all phases of the endodontic retreatment, regardless of the method used for microbial identification. Associations were found between specific bacteria and clinical/radiographic features. (AU)

• Processo FAPESP: 17/25242-8 - Monitoramento clínico, microbiológico e imunológico de pacientes submetidos à terapia endodôntica com diagnóstico de Pulpite irreversível
• Beneficiário: Rodrigo Arruda Vasconcelos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/23479-5 - Microbiomas e aspectos imunobiológicos nas infecções endodônticas
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/10755-5 - Investigação clínica de modulações epigenéticas em polpas dentais com diagnóstico de pulpite irreversível
• Beneficiário: Rodrigo Arruda Vasconcelos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 19/19300-0 - Investigação dos aspectos clínicos, microbiológicos e imunológicos de dentes com diagnóstico de lesão periodontal primária com envolvimento endodôntico secundário
• Beneficiário: Lidiane Mendes Louzada
• Modalidade de apoio: Bolsas no Brasil - Doutorado