Argon Atmospheric Plasma Treatment Promotes Burn Healing by Stimulating Inflammation and Controlling the Redox State

Autor(es):Mostrar menos -

de Souza, Lucas Buzeli ^[1] ; de Souza Silva, Jennyffer Ione ^[1] ; Bagne, Leonardo ^[1] ; Pereira, Amanda Tavares ^[1] ; de Oliveira, Maraiara Aparecida ^[1] ; Lopes, Bruno Bellotti ^[2] ; Corezola do Amaral, Maria Esmeria ^[1] ; de Aro, Andrea Aparecida ^[1] ; Marretto Esquisatto, Marcelo Augusto ^[1] ; Tech dos Santos, Glaucia Maria ^[1] ; Moretti de Andrade, Thiago Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Ctr Herminio Ometto Fdn FHO, Grad Program Biomed Sci, Dr Maximiliano Baruto Ave 500, BR-13607339 Araras, SP - Brazil

^[2] Surface Engn & Solut Plasma Ltda, Jose Rocha Bonf Ave, 214 Lot Ctr Santa Genebra, BR-13080658 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Inflammation; v. 43, n. 6 AUG 2020.
• Citações Web of Science: 0

Resumo

Burns are a public health problem, with second-degree burns as one of the most common types. Although intense inflammation worsens burn healing, effective therapies are scarce. Thus, infections and hypertrophic scars may occur, which compromise patient quality of life and may delay healing. Argon atmospheric plasma (AP) has been shown to positively influence wound healing. In the context of identifying effective and alternative therapies for the treatment of second-degree burns, the present study evaluated AP in the treatment of second-degree burns in rats compared to that for sham treatment on the 2nd, 7th, 14th, and 21st days post-injury. Our results revealed proinflammatory effect for AP by recruiting predominantly neutrophils on the 7th day and macrophages on the 21st day compared to sham treatment, allowing a greater production of interleukin (IL)-1 beta, tumor necrosis factor (TNF)-alpha, and IL-17, and also controlled the inflammation by IL-10 and transforming growth factor (TGF)-beta 1. AP also showed antioxidant activity important for controlling oxidative damage on the 2nd day. This favored the induction of angiogenesis from the 2nd day and induction fibroplasia and fibrillogenesis after the 14th day, which enhanced burn healing with the formation of a thinner burn eschar before the 21st day post-burn. Thus, AP effectively modulated the inflammatory phase of second-degree burn healing through the control of oxidative damage that favored the following phases. Therefore, AP is a relevant alternative in the treatment of second-degree burns. (AU)

• Processo FAPESP: 17/00126-5 - Estudo do plasma atmosférico de argônio na cicatrização de úlceras cutâneas por excisão e queimadura em ratos induzidos ao diabetes
• Beneficiário: Bruno Bellotti Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE