NMR-based metabolic profiling to follow the production of anti- phytopathogenic compounds in the culture of the marine strain Streptomyces sp. PNM-9

Autor(es)

Betancur, Luz A. ^{[1, 2]} ; Forero, Abel M. ^[1] ; Vinchira-Villarraga, Diana M. ^[1] ; Cardenas, Juan D. ^[1] ; Romero-Otero, Adriana ^[1] ; Chagas, Fernanda O. ^{[3, 4]} ; Pupo, Monica T. ^[3] ; Castellanos, Leonardo ^[1] ; Ramos, Freddy A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Nacl Colombia, Dept Quim, Sede Bogota, Carrera 30 45-03, Edificio Quim 427, Bogota 111321 - Colombia

^[2] Univ Caldas, Dept Quim, Edificio Orlando Sierra, Bloque B, Manizales, Caldas - Colombia

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Fed Univ Rio de Janeiro IPPN UFRJ, Walter Mors Inst Res Nat Prod, Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROBIOLOGICAL RESEARCH; v. 239, OCT 2020.
• Citações Web of Science: 0

Resumo

Actinobacteria are the major source of bioactive secondary metabolites and are featured in the search for antimicrobial compounds. We used nuclear magnetic resonance (RMN)-metabolic profiling and multivariate data analysis (MVDA) to correlate the metabolites' production of Streptomyces sp. PNM-9 from the algae Dictyota sp. and their biological activity against the rice phytopathogenic bacteria Burkholderia spp. The compounds 2-methyl-N-(2 `-phenylethyl)-butanamide (1) and 3-methyl-N-(2 `-phenylethyl)-butanamide (2) were identified through MVDA and 2D NMR experiments in the organic extract of a 15-days LB media culture of Streptomyces sp. PNM-9. Compounds 1 and 2 were isolated and their structures confirmed by oneand two-dimensional NMR and mass spectrometry (MS) data. Compounds 1 and 2 were active against the rice pathogenic bacteria Burkholderia glumae (ATCC 33,617) displaying minimal inhibitory concentration (MIC) values of 2.43 mM and 1.21 mM, respectively. The metabolomics-guided approach employing NMR-metabolic profiling was useful for marine microbial bioprospecting and suggested Streptomyces sp. PNM-9 strain and its compounds as a potential control against phytopathogenic bacteria. (AU)

• Processo FAPESP: 13/50954-0 - Novos agentes terapêuticos obtidos de bactérias simbiontes de invertebrados brasileiros
• Beneficiário: Mônica Tallarico Pupo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático