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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

NMR-based metabolic profiling to follow the production of anti- phytopathogenic compounds in the culture of the marine strain Streptomyces sp. PNM-9

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Author(s):
Betancur, Luz A. [1, 2] ; Forero, Abel M. [1] ; Vinchira-Villarraga, Diana M. [1] ; Cardenas, Juan D. [1] ; Romero-Otero, Adriana [1] ; Chagas, Fernanda O. [3, 4] ; Pupo, Monica T. [3] ; Castellanos, Leonardo [1] ; Ramos, Freddy A. [1]
Total Authors: 9
Affiliation:
[1] Univ Nacl Colombia, Dept Quim, Sede Bogota, Carrera 30 45-03, Edificio Quim 427, Bogota 111321 - Colombia
[2] Univ Caldas, Dept Quim, Edificio Orlando Sierra, Bloque B, Manizales, Caldas - Colombia
[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[4] Fed Univ Rio de Janeiro IPPN UFRJ, Walter Mors Inst Res Nat Prod, Rio De Janeiro, RJ - Brazil
Total Affiliations: 4
Document type: Journal article
Source: MICROBIOLOGICAL RESEARCH; v. 239, OCT 2020.
Web of Science Citations: 0
Abstract

Actinobacteria are the major source of bioactive secondary metabolites and are featured in the search for antimicrobial compounds. We used nuclear magnetic resonance (RMN)-metabolic profiling and multivariate data analysis (MVDA) to correlate the metabolites' production of Streptomyces sp. PNM-9 from the algae Dictyota sp. and their biological activity against the rice phytopathogenic bacteria Burkholderia spp. The compounds 2-methyl-N-(2 `-phenylethyl)-butanamide (1) and 3-methyl-N-(2 `-phenylethyl)-butanamide (2) were identified through MVDA and 2D NMR experiments in the organic extract of a 15-days LB media culture of Streptomyces sp. PNM-9. Compounds 1 and 2 were isolated and their structures confirmed by oneand two-dimensional NMR and mass spectrometry (MS) data. Compounds 1 and 2 were active against the rice pathogenic bacteria Burkholderia glumae (ATCC 33,617) displaying minimal inhibitory concentration (MIC) values of 2.43 mM and 1.21 mM, respectively. The metabolomics-guided approach employing NMR-metabolic profiling was useful for marine microbial bioprospecting and suggested Streptomyces sp. PNM-9 strain and its compounds as a potential control against phytopathogenic bacteria. (AU)

FAPESP's process: 13/50954-0 - Novel therapeutic agents from the bacterial symbionts of Brazilian invertebrates
Grantee:Mônica Tallarico Pupo
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants