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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Assessment of the antibacterial, antivirulence, and action mechanism of Copaifera pubiflora oleoresin and isolated compounds against oral bacteria

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Autor(es):
Moraes, Thais da Silva [1] ; Leandro, Luis Fernando [1] ; Santiago, Mariana Brentini [2] ; Silva, Larissa de Oliveira [1] ; Bianchi, Thamires Chiquini [1] ; Veneziani, Rodrigo Cassio Sola [1] ; Ambrosio, Sergio Ricardo [1] ; Ramos, Salvador Boccaletti [3] ; Bastos, Jairo Kenupp [4] ; Martins, Carlos Henrique Gomes [1, 2]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Franca, Lab Res Appl Microbiol LaPeMA, BR-14404600 Franca, SP - Brazil
[2] Univ Fed Uberlandia, Inst Biomed Sci, Lab Antimicrobial Testing LEA, 1720 Para Ave, Block 2B Room 2B221, BR-38405320 Uberlandia, MG - Brazil
[3] Univ Franca, Postgrad Program Hlth Promot, BR-14404600 Franca, SP - Brazil
[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: BIOMEDICINE & PHARMACOTHERAPY; v. 129, SEP 2020.
Citações Web of Science: 3
Resumo

The microorganisms that constitute the oral microbiome can cause oral diseases, including dental caries and endodontic infections. The use of natural products could help to overcome bacterial resistance to the anti-microbials that are currently employed in clinical therapy. This study assessed the antimicrobial activity of the Copaifera pubiflora oleoresin and of the compounds isolated from this resin against oral bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays provided values ranging from 6.25 to > 400 mu g/mL for the C. pubiflora oleoresin and its isolated compounds. The fractional inhibitory concentration index (FICI) assay showed that the oleoresin and chlorhexidine did not act synergistically. All the tested bacterial strains formed biofilms. MICB(50)( )determination revealed inhibitory action: values varied from 3.12-25 mu g/mL for the oleoresin, and from 0.78 to 25 mu g/mL for the ent-hardwickiic acid. Concerning biofilm eradication, the C. pubiflora oleoresin and hardwickiic acid eradicated 99.9 % of some bacterial biofilms. Acid resistance determination showed that S. mutans was resistant to acid in the presence of the oleoresin and enthardwickiic acid at pH 4.0, 4.5, and 5.0 at all the tested concentrations. Analysis of DNA/RNA and protein release by the cell membrane demonstrated that the oleoresin and hardwiickic acid damaged the bacterial membrane irreversibly, which affected membrane integrity. Therefore, the C. pubiflora oleoresin and ent-hardwickiic acid have potential antibacterial effect and can be used as new therapeutic alternatives to treat oral diseases such as dental caries and endodontic infections. (AU)

Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
Beneficiário:Jairo Kenupp Bastos
Modalidade de apoio: Auxílio à Pesquisa - Temático