Polymorphisms in major histocompatibility complex genes and its associations with milk quality in Murrah buffaloes

Autor(es)

Freitas, Ana C. ^[1] ; Stafuzza, Nedenia B. ^[2] ; Barbero, Marina M. D. ^[1] ; Santos, Daniel J. A. ^[1] ; Fortes, Marina R. S. ^[3] ; Tonhati, Humberto ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci FCAV, Dept Anim Sci, BR-14884900 Jaboticabal, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci FCAV, Dept Exact Sci, BR-14884900 Jaboticabal, SP - Brazil

^[3] Univ Queensland, Sch Chem & Mol Biosci, St Lucia Campus, Brisbane, Qld 4067 - Australia

• Tipo de documento: Artigo Científico
• Fonte: TROPICAL ANIMAL HEALTH AND PRODUCTION; v. 52, n. 1, p. 415-423, JAN 2020.
• Citações Web of Science: 0

Resumo

Animal breeding programs have used molecular genetic tools as an auxiliary method to identify and select animals with superior genetic merit for milk production and milk quality traits as well as disease resistance. Genes of the major histocompatibility complex (MHC) are important molecular markers for disease resistance that could be applied for genetic selection. The aim of this study was to identify single nucleotide polymorphisms (SNPs) and haplotypes in DRB2, DRB3, DMA, and DMB genes in Murrah breed and to analyze the association between molecular markers and milk, fat, protein and mozzarella production, fat and protein percentage, and somatic cell count. Two hundred DNA samples from Murrah buffaloes were used. The target regions of candidate genes were amplified by polymerase chain reaction (PCR) followed by sequencing and identification of polymorphisms. Allele and genotype frequencies, as well as linkage disequilibrium between SNPs, were calculated. Genotypes were used in association analyses with milk production and quality traits. Except for the DMA gene, identified as monomorphic, the other genes presented several polymorphisms. The DMB, DRB2, and DRB3 genes presented two, six, and seven SNPs, respectively. Fifty-seven haplotype blocks were constructed from 15 SNPs identified, which was used in association analyses. All the studied traits had at least one associated haplotype. In conclusion, it is suggested that the haplotypes found herein can be associated with important traits related to milk production and quality. (AU)

• Processo FAPESP: 15/12396-1 - Imputação de genótipos de marcadores no genoma bubalino e seu impacto nas avaliações genômicas da produção de leite e seus constituintes
• Beneficiário: Daniel Jordan de Abreu Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/25309-7 - Polimorfismos e expressão de genes do sistema imune associados à mastite em búfalas leiteiras
• Beneficiário: Humberto Tonhati
• Modalidade de apoio: Auxílio à Pesquisa - Regular