Calcium and Phosphorus Levels in Saliva are Influenced by Genetic Polymorphisms in Estrogen Receptor Alpha and Microrna17

Autor(es):Mostrar menos -

Erika Calvano Küchler ^[1] ; Raquel Fernanda Gerlach ^[2] ; Arthur S Cunha ^[3] ; Lucas A Ramazzotto ^[4] ; Paula Porto Spada ^[5] ; Paulo Nelson-Filho ^[6] ; Junia Ramos ^[7] ; Giovana Daniela Pecharki ^[8] ; Fernando Barbosa Jr ^[9] ; Alexandre Rezende Vieira ; Paula Cristina Trevilatto ^[11] ; João Armando Brancher ^[12]

Afiliação do(s) autor(es):Mostrar menos -

^[1] Universidade de São Paulo. Ribeirão Preto Dental School. Department of Pediatric Dentistry - Brasil

^[2] Universidade de São Paulo. Physiology and Basic Pathology, Ribeirão Preto Dental School. Department of Morphology - Brasil

^[3] Universidade de São Paulo. Ribeirão Preto Dental School. Department of Pediatric Dentistry - Brasil

^[4] Universidade de São Paulo. Ribeirão Preto Dental School. Department of Pediatric Dentistry - Brasil

^[5] Universidade Positivo. School of Health Sciences - Brasil

^[6] Universidade de São Paulo. Ribeirão Preto Dental School. Department of Pediatric Dentistry - Brasil

^[7] Universidade de São Paulo. Physiology and Basic Pathology, Ribeirão Preto Dental School. Department of Morphology - Brasil

^[8] Universidade Federal do Paraná. Department of Community Health - Brasil

^[9] Universidade de São Paulo. Faculty of Pharmaceutical Sciences of Ribeirao Preto. Department of Clinical, Toxicological and Bromatological Analysis - Brasil

^[11] Pontifícia Universidade Católica do Paraná. Center for Health and Biological Sciences - Brasil

^[12] Universidade Positivo. School of Health Sciences - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 31, n. 5, p. 466-470, 2020-11-02.

Resumo

Abstract Homeostasis between salivary calcium and phosphorus is important for maintaining oral health. The aim of this study was to evaluate if polymorphisms in ESR1 (Estrogen Receptor Alpha), ESR2 (Estrogen Receptor Beta) and miRNA17 (microRNA17) are associated with calcium and phosphorus levels in saliva. Saliva from 276 12-year-old children were collected by masticatory stimulation and calcium and phosphorus levels were determined by Mass Spectrometry. Genomic DNA was extracted from remaining saliva and genetic polymorphisms in ESR1 (rs12154178, rs1884051, rs9340799 and rs2234693), in ESR2 (rs4986938 and rs1256049) and in miRNA17 (rs4284505) were genotyped using TaqMan chemistry and a real-time PCR equipment. Statistical differences in genotype and allele distributions between ‘low’ and ‘high’ calcium and phosphorus levels were determined using chi-square or Fisher´s exact tests. The analysis was also adjusted by sex (alpha of 5%). ESR1 rs9340799 had the less common genotype associated with higher calcium levels (p=0.03). The less common allele of ESR1 rs1884051 was associated with lower phosphorus levels (p=0.005) and there was an excess of heterozygotes for miRNA17 rs4284505 among individuals with lower calcium levels (p=0.002), both adjusted by sex. This study provides evidence that genetic polymorphisms in ESR1 and miRNA17 are involved in determining salivary calcium and phosphorus levels. (AU)

• Processo FAPESP: 15/06866-5 - Avaliação do papel do estrógeno no desenvolvimento dentofacial
• Beneficiário: Erika Calvano Kuchler
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/08149-1 - Avaliação do papel do estrógeno nos tecidos dentofaciais
• Beneficiário: Erika Calvano Kuchler
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores