Lack of dystrophin influences muscle inflammation but not myogenic regulatory factors after eccentric exercise in mdx mice

Autor(es)

Mariana Cruz Lazzarin ^[1] ; Hananiah Tardivo Quintana ^[2] ; Vivianne Izabelle de Araújo Baptista ^[3] ; Flavia de Oliveira ^[4]

Afiliação do(s) autor(es)

^[1] Universidade Federal de São Paulo. Departamento de Biociências. Programa de Pós-graduação Interdisciplinar em Ciências da Saúde - Brasil

^[2] Universidade Federal de São Paulo. Departamento de Biociências. Programa de Pós-graduação Interdisciplinar em Ciências da Saúde - Brasil

^[3] Universidade Federal do Rio Grande do Norte. Escola Multicampi de Ciências Medicas - Brasil

^[4] Universidade Federal de São Paulo. Departamento de Biociências. Programa de Pós-graduação Interdisciplinar em Ciências da Saúde - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Motriz: rev. educ. fis.; v. 26, n. 3 2020-12-02.

Resumo

Abstract Aim: To investigate the consequences of chronic eccentric exercise in histopathology, inflammatory, and myogenic regulatory factors response in gastrocnemius muscle of X-chromosome-linked muscular dystrophy (mdx) mice. Method: Male mdx and control mice (C57BL/10 lineage) were distributed in the following groups: Sedentary Control (SC), Trained Control (TC), Sedentary Mdx (S-Mdx), and Trained Mdx (T-Mdx). Trained animals were subjected to downhill running for 7 weeks. Gastrocnemius was submitted to histopathological analysis and immunoexpression of Cyclooxygenase-2 (COX-2) and myogenic regulatory factors (myoD and myogenin). Results: The exercise influenced inflammation response as demonstrated by the increased COX-2 immunoexpression in T-Mdx. Interestingly, Myogenic regulatory factors revealed that the lack of dystrophin has not been influenced myoD and the increase of myogenin occurred due to exercise and was not aggravated by the absence of dystrophin. Conclusion: In conclusion, an eccentric exercise in gastrocnemius of mdx mice was characterized by an intense inflammatory process without myogenic response. These findings suggest that special attention should be given to inflammatory aspects related to COX-2 associated with a decrease of myoD expression, as biomarkers in motor rehabilitation programs. (AU)

• Processo FAPESP: 14/22211-6 - Características do processo de regeneração de músculos esqueléticos de diferentes origens embrionárias em camundongos mdx submetidos ao treinamento físico aeróbio
• Beneficiário: Mariana Cruz Lazzarin
• Modalidade de apoio: Bolsas no Brasil - Mestrado