Strength exercise reduces hepatic pyruvate carboxylase and gluconeogenesis in DIO mice

Autor(es):Mostrar menos -

Pereira, Rodrigo Martins ^[1] ; da Cruz Rodrigues, Kellen Cristina ^[1] ; Sant'Ana, Marcella Ramos ^[2] ; Peruca, Guilherme Francisco ^[1] ; Morelli, Ana Paula ^[3] ; Simabuco, Fernando M. ^[3] ; da Silva, Adelino S. R. ^{[4, 5]} ; Cintra, Dennys Esper ^[2] ; Ropelle, Eduardo Rochete ^[6] ; Pauli, Jose Rodrigo ^[6] ; de Moura, Leandro Pereira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Appl Sci, Exercise Cell Biol Lab, Limeira - Brazil

^[2] Univ Estadual Campinas, Fac Appl Sci, Lab Nutr Genom, Limeira - Brazil

^[3] Univ Estadual Campinas, Fac Appl Sci, Multidisciplinary Lab Food & Hlth, Limeira - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil

^[6] State Univ Campinas UNICAMP, Fac Appl Sci, Lab Mol Biol Exercise LaBMEx, Limeira, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Endocrinology; v. 247, n. 2, p. 127-138, NOV 2020.
• Citações Web of Science: 0

Resumo

Obesity is linked to a reduction in the control of hepatic glucose production, which is the primary mechanism related to fasting hyperglycemia and the development of type 2 diabetes mellitus (T2DM). The main system involved in hepatic gluconeogenesis synthesis is controlled by pyruvate carboxylase (PC), which increases in obesity conditions. Recently, we showed that short-term strength training is an important tool against obesity-induced hyperglycemia. As aerobic exercise can reduce the hepatic PC content of obese animals, we hypothesized that strength exercise can also decrease this gluconeogenic enzyme. Therefore, this study investigated whether the metabolic benefits promoted by short-term strength training are related to changes in hepatic PC content. Swiss mice were divided into three groups: lean control (Ctl), obese sedentary (ObS), and obese short-term strength training (STST). The STST protocol was performed through one session/day for 15 days. The obese exercised animals had reduced hyperglycemia and insulin resistance. These results were related to better control of hepatic glucose production and hepatic insulin sensitivity. Our bioinformatics analysis showed that hepatic PC mRNA levels have positive correlations with glucose levels and adiposity, and negative correlations with locomotor activity and muscle mass. We also found that hepatic mRNA levels are related to lipogenic markers in the liver. Finally, we observed that the obese animals had an increased hepatic PC level; however, STST was efficient in reducing its amount. In conclusion, we provide insights into new biomolecular mechanisms by showing how STST is an efficient tool against obesityrelated hyperglycemia and T2DM, even without body weight changes. (AU)

• Processo FAPESP: 16/12569-6 - Efeitos de diferentes intensidades do exercício físico no metabolismo da clusterina e sua interferência na sinalização da insulina em roedores
• Beneficiário: Rodrigo Martins Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/07199-2 - Papel da clusterina/ApoJ na sinalização da insulina em resposta ao exercício físico em roedores e em humanos
• Beneficiário: Leandro Pereira de Moura
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/24406-4 - O papel do exercício físico na via de sinalização molecular da clusterina/leptina em hipotálamo de camundongos obesos
• Beneficiário: Kellen Cristina da Cruz Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto