Uncaria tomentosa reduces osteoclastic bone loss in vivo

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Lima, Vilma ^{[1, 2, 3]} ; Melo, Iracema Matos ^[4] ; Taira, Thaise Mayumi ^[3] ; Wilches Buitrago, Liseth Yamile ^[3] ; Roriz Fonteles, Cristiane Sa ^[4] ; Almeida Moreira Leal, Luzia Kalyne ^[5] ; de Queiroz Souza, Ana Sheila ^[5] ; Almeida, Talysson Silva ^[5] ; da Costa Filho, Raimundo Nogueira ^[6] ; Moraes, Manoel Odorico ^[2] ; Cunha, Fernando Queiroz ^[1] ; Fukada, Sandra Yasuyo ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pharmacol, Ribeirao Preto - Brazil

^[2] Univ Fed Ceara, Dept Physiol & Pharmacol, Sch Med, Fortaleza, Ceara - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Biomol Sci, Ave Cafe S-N Bloco S, 3 Andar, Sala 90A-S, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Univ Fed Ceara, Sch Pharm, Sch Pharm Nursing & Dent, Fortaleza, Ceara - Brazil

^[5] Univ Fed Ceara, Dept Pharm, Sch Pharm Nursing & Dent, Fortaleza, Ceara - Brazil

^[6] Univ Fed Ceara, Dept Phys, Fortaleza, Ceara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Phytomedicine; v. 79, DEC 2020.
• Citações Web of Science: 0

Resumo

Background: The genus Uncaria (Rubiaceae) has several biological properties significant to human health. However, the mechanisms underlying the protective effect of this plant on bone diseases are uncertain. Purpose: The present study investigated the role of Uncaria tomentosa extract (UTE) on alveolar bone loss in rats and on osteoclastogenesis in vitro. Materials: UTE was characterized by an Acquity UPLC (Waters) system, coupled to an Electrospray Ionization (ESI) interface and Quadrupole/Flight Time (QTOF, Waters) Mass Spectrometry system (MS). The effect of UTE treatment for 11 days on the ligature-induced bone loss was assessed focusing on several aspects: macroscopic and histological analysis of bone loss, neutrophil and osteoclast infiltration, and anabolic effect. The effect of UTE on bone marrow cell differentiation to osteoclasts was assessed in vitro. Results: The analysis of UTE by UPLC-ESI-QTOF-MS/MS identified 24 compounds, among pentacyclic or tetracyclic oxindole alkaloids and phenols. The administration of UTE for 11 days on ligature-induced rat attenuated the periodontal attachment loss and alveolar bone resorption. It also diminished neutrophil migration to the gingiva tissue, demonstrated by a lower level of MPO. UTE treatment also decreased the level of RANKL/OPG ratio, the main osteoclast differentiation-related genes, followed by reduced TRAP-positive cell number lining the alveolar bone. Additionally, the level of bone-specific alkaline phosphatase, an anabolic bone marker, was elevated in the plasma of UTE treated rats. Next, we determined a possible direct effect of UTE on osteoclast differentiation in vitro. The incubation of primary osteoclast with UTE decreased RANKL-induced osteoclast differentiation without affecting cell viability. This effect was supported by downregulation of the nuclear factor activated T-cells, cytoplasmic 1 expression, a master regulator of osteoclast differentiation, and other osteoclast-specific activity markers, such as cathepsin K and TRAP. Conclusion: UTE exhibited an effective anti-resorptive and anabolic effects, which highlight it as a potential natural product for the treatment of certain osteolytic diseases, such as periodontitis. (AU)

• Processo FAPESP: 15/09034-0 - Efeito da O-GlcNacilação sobre a osteoclastogênese e reabsorção óssea
• Beneficiário: Sandra Yasuyo Fukada Alves
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs