| Full text | |
| Author(s): Show less - |
Lima, Vilma
[1, 2, 3]
;
Melo, Iracema Matos
[4]
;
Taira, Thaise Mayumi
[3]
;
Wilches Buitrago, Liseth Yamile
[3]
;
Roriz Fonteles, Cristiane Sa
[4]
;
Almeida Moreira Leal, Luzia Kalyne
[5]
;
de Queiroz Souza, Ana Sheila
[5]
;
Almeida, Talysson Silva
[5]
;
da Costa Filho, Raimundo Nogueira
[6]
;
Moraes, Manoel Odorico
[2]
;
Cunha, Fernando Queiroz
[1]
;
Fukada, Sandra Yasuyo
[3]
Total Authors: 12
|
| Affiliation: | [1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Pharmacol, Ribeirao Preto - Brazil
[2] Univ Fed Ceara, Dept Physiol & Pharmacol, Sch Med, Fortaleza, Ceara - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Biomol Sci, Ave Cafe S-N Bloco S, 3 Andar, Sala 90A-S, BR-14040903 Ribeirao Preto, SP - Brazil
[4] Univ Fed Ceara, Sch Pharm, Sch Pharm Nursing & Dent, Fortaleza, Ceara - Brazil
[5] Univ Fed Ceara, Dept Pharm, Sch Pharm Nursing & Dent, Fortaleza, Ceara - Brazil
[6] Univ Fed Ceara, Dept Phys, Fortaleza, Ceara - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Phytomedicine; v. 79, DEC 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Background: The genus Uncaria (Rubiaceae) has several biological properties significant to human health. However, the mechanisms underlying the protective effect of this plant on bone diseases are uncertain. Purpose: The present study investigated the role of Uncaria tomentosa extract (UTE) on alveolar bone loss in rats and on osteoclastogenesis in vitro. Materials: UTE was characterized by an Acquity UPLC (Waters) system, coupled to an Electrospray Ionization (ESI) interface and Quadrupole/Flight Time (QTOF, Waters) Mass Spectrometry system (MS). The effect of UTE treatment for 11 days on the ligature-induced bone loss was assessed focusing on several aspects: macroscopic and histological analysis of bone loss, neutrophil and osteoclast infiltration, and anabolic effect. The effect of UTE on bone marrow cell differentiation to osteoclasts was assessed in vitro. Results: The analysis of UTE by UPLC-ESI-QTOF-MS/MS identified 24 compounds, among pentacyclic or tetracyclic oxindole alkaloids and phenols. The administration of UTE for 11 days on ligature-induced rat attenuated the periodontal attachment loss and alveolar bone resorption. It also diminished neutrophil migration to the gingiva tissue, demonstrated by a lower level of MPO. UTE treatment also decreased the level of RANKL/OPG ratio, the main osteoclast differentiation-related genes, followed by reduced TRAP-positive cell number lining the alveolar bone. Additionally, the level of bone-specific alkaline phosphatase, an anabolic bone marker, was elevated in the plasma of UTE treated rats. Next, we determined a possible direct effect of UTE on osteoclast differentiation in vitro. The incubation of primary osteoclast with UTE decreased RANKL-induced osteoclast differentiation without affecting cell viability. This effect was supported by downregulation of the nuclear factor activated T-cells, cytoplasmic 1 expression, a master regulator of osteoclast differentiation, and other osteoclast-specific activity markers, such as cathepsin K and TRAP. Conclusion: UTE exhibited an effective anti-resorptive and anabolic effects, which highlight it as a potential natural product for the treatment of certain osteolytic diseases, such as periodontitis. (AU) | |
| FAPESP's process: | 15/09034-0 - The role of O-glycosylation on the modulation of osteoclastogenesis and bone resorption |
| Grantee: | Sandra Yasuyo Fukada Alves |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/08216-2 - CRID - Center for Research in Inflammatory Diseases |
| Grantee: | Fernando de Queiroz Cunha |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |