The effect of association of low-level laser irradiation and poly(vinylidene-trifluoroethylene)/barium titanate composite in the repair of bone defects in calvaria of ovariectomized rats.

• Grant number: 17/25683-4
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2018
• End date: May 31, 2020
• Field of knowledge: Biological Sciences - Morphology - Histology
• Principal Investigator: Selma Siéssere
• Grantee: Selma Siéssere
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Adalberto Luiz Rosa ; Karina Fittipaldi Bombonato Prado ; Márcio Mateus Beloti ; Rossano Gimenes ; Simone Cecilio Hallak Regalo

Abstract

Osteoporosis is a disease that compromises the strength and quality of bone tissue. Different methodologies in the field of dentistry have been used in search of bone repair in the face of this disease that can predispose to periodontal diseases, accelerating the loss of alveolar bone. Low-level laser therapy is a methodology that has been used in the search for new bone formation. Different times, number of applications, wavelengths and energy densities have been studied. Another methodology is guided tissue regeneration. In vitro and in vivo experiments have demonstrated favorable results of the use of the membrane obtained by the association of poly(vinylidene-trifluoroethylene) polymer and barium titanate ceramics (P (VDF-TrFE) / BT) when compared to the polytetrafluoroethylene membrane (PTFE). Thus, this study aims to evaluate in vivo the response of bone tissue to low-level laser irradiation when associated with PTFE (control) and P(VDF-TrFE)/BT membranes in bone defects in calvaria of rats with experimental model to osteoporosis. Female Wistar rats (300g) will undergo the simulation of ovariectomy surgery (Group 1 - Sham; n=5) and will be bilaterally ovariectomized (n=35). After 90 days, bone defects (5 mm) will be performed on calvaria and the ovariectomized animals will be divided into four groups: Group 2 - without membrane and without laser application (n=5); - Group 3 - P(VDF-TrFE)/BT membrane (n=10); Group 4 - P(VDF-TrFE)/BT membrane + laser application (n=10); Group 5 - PTFE membrane + laser application (n=10). The gallium-aluminum arsenide (GaAlAs) diode will be used with a wavelength of 780 nm and energy density or fluence of 30J/cm2 (12 sessions). At the end of thirty days after bone defect creation, the animals will be sacrificed and will be carried out as follows: (1) tomographic analysis (micro-CT) of the defects previously created, (2) histological analysis in non-decalcified histological sections, (3) the mRNA expression of runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OC), osteopontin (OPN), osterix (OSX), receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), receptor activator of nuclear factor-kappa B (RANK), Cathepsin K and matrix metalloproteinase-9 (MMP-9) by real-time PCR. Statistical tests to analyze the numerical results will be selected according to the distribution of samples. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TOLOI RUFATO, FERNANDA CRISTINA; DE SOUSA, LUIZ GUSTAVO; SCALIZE, PRISCILLA HAKIME; GIMENES, ROSSANO; REGALO, ISABELA HALLAK; ROSA, ADALBERTO LUIZ; BELOTI, MARCIO MATEUS; DE OLIVEIRA, FABIOLA SINGARETTI; BOMBONATO-PRADO, KARINA FITTIPALDI; HALLAK REGALO, SIMONE CECILIO; et al. Texturized P(VDF-TrFE)/BT membrane enhances bone neoformation in calvaria defects regardless of the association with photobiomodulation therapy in ovariectomized rats. CLINICAL ORAL INVESTIGATIONS, v. 26, n. 1, AUG 2021. (17/25683-4)

RUFATO, F. T. F.; SOUSA, L. G.; SCALIZE, P. H.; BELOTI, M. M.; ALMEIDA, A. L. G.; GIMENES, R.; BIANCO, S. C.; BOMBONATO-PRADO, K. F.; REGALO, S. C. H.; SIESSERE, S.. MICROTOMOGRAPHIC EVALUATION OF BONE NEOFORMATION IN CALVARIA BONE DEFECTS AFTER THE ASSOCIATION OF PVDF MEMBRANE AND PHOTOBIOMODULATION THERAPY IN OVARIECTOMIZED RATS. OSTEOPOROSIS INTERNATIONAL, v. 31, n. SUPPL 1, p. 1-pg., 2020-12-01. (17/25683-4)

SIESSERE, S.; RUFATO, F. T. F.; SOUSA, L. G.; SCALIZE, P. H.; RICARDO, V.; ROSA, A. L.; OLIVEIRA, F. S.; GIMENES, R.; BOMBONATO-PRADO, K. F.; REGALO, S. C. H.. QUANTITATIVE GENE EXPRESSION IN CALVARIA BONE CELLS AFTER PHOTOBIOMODULATION THERAPY ASSOCIATED WITH MEMBRANES IN OVARIECTOMIZED RATS. OSTEOPOROSIS INTERNATIONAL, v. 31, n. SUPPL 1, p. 2-pg., 2020-12-01. (17/25683-4)