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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Leishmania amazonensis Promastigotes or Extracellular Vesicles Modulate B-1 Cell Activation and Differentiation

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Autor(es):
Reis, Natasha Ferraz de Campos [1] ; Dupin, Talita Vieira [1] ; Costa, Carolina Rizzaro [1] ; Toledo, Mayte dos Santos [1] ; de Oliveira, Vivian Cristina [2] ; Popi, Ana Flavia [2] ; Torrecilhas, Ana Claudia [1] ; Xander, Patricia [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Dept Pharmaceut Sci, Lab Cellular Immunol & Biochem Fungi & Protozoa, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Paulista Sch Med, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 10, OCT 30 2020.
Citações Web of Science: 0
Resumo

B-1 cells are considered an innate-like B cell population that participates in effective innate and adaptive responses to pathogens. B-1 cells produce immunoglobulins, cytokines, chemokines, migrate to inflammatory sites, and differentiate into mononuclear phagocyte-like cells. Murine B-1 cells phagocytosed Leishmania in vitro and in vivo and participate in immunity against Leishmania. Our group showed that B-1 cells or their extracellular vesicles (EVs) led to a resistance to experimental infection by L. amazonensis. However, the B-1 cells' responses to Leishmania or EVs isolated from parasites are still poorly characterized. Studying the activation and differentiation of B-1 cells in vivo can contribute to a better understanding of how these cells participate in immunity to L. amazonensis. Thus, we evaluated the expression of myeloid (M-csfr, G-csfr, Spi-1) and lymphoid (EBF, E2A, IL-7R) lineage commitment factors, Toll-like receptors (TLRs), activation cell surface markers, nitric oxide (NO) and reactive oxygen species (ROS) production in murine peritoneal B-1 cells collected after 24 or 48 h post-infection with Leishmania (Leishmania) amazonensis promastigotes or EVs released by the parasites. Our results demonstrated that L. amazonensis infection did not stimulate the expression of CD40, CD80, CD86, F4/80, and MHC II in B-1 cells, but a significant decrease in the production of NO and ROS was observed. The infection induced a significantly higher arginase expression in B-1 cells, but the stimulation with EVs led to a decrease in this gene expression. TLR-2 and TLR-6 had significantly higher expression in B-1 cells from mice intraperitoneally stimulated with the parasite. The TLR-9 expression was higher in animals infected or stimulated for 48 h with EVs. Interestingly, in B-1 cells the stimulus with L. amazonensis led to a substantial increase in the expression of myeloid restricted transcription factors. Thus, our study suggests that the parasites or EVs differently modulated the activation and differentiation of B-1 cells. (AU)

Processo FAPESP: 16/17245-4 - Avaliação dos efeitos das vesículas extracelulares liberadas por promastigotas de Leishmania amazonensis na resposta de macrófagos e na progressão da doença
Beneficiário:Patricia Xander Batista
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 19/21614-3 - Vesículas extracelulares liberadas por Leishmania (Leishmania) amazonensis com distintos perfis de virulência: caracterização, papel na resposta imunológica e na progressão da doença
Beneficiário:Patricia Xander Batista
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/06597-2 - Avaliação dos efeitos das vesículas extracelulares liberadas por promastigotas de Leishmania amazonensis na resposta de macrófagos e na progressão da doença
Beneficiário:Natasha Ferraz de Campos Reis
Linha de fomento: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico