| Texto completo | |
| Autor(es): |
Aguiar, Weslei da Silva
[1, 2]
;
Galizio, Nathalia da Costa
[1, 2]
;
Sant'Anna, Savio Stefanini
[2]
;
Silveira, Giovanni Perez M.
[2]
;
Rodrigues, Fabiola de Souza
[2]
;
Grego, Kathleen Fernandes
[2]
;
De Morais-Zani, Karen
[1, 2]
;
Tanaka-Azevedo, Anita Mitico
[1, 2]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Pesquisas Tecnol, Inst Butantan, Interunidades Biotecnol, Sao Paulo - Brazil
[2] Inst Butantan, Lab Herpetol, Av Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Toxicon; v. 186, p. 67-77, OCT 30 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Concerning snake venoms, numerous authors worked with different species of Bothrops focusing on the ontogeny of these animals. However, according to PubMed database, no results on studies related to Bothrops jararacussu ontogeny were displayed until now. This fact led us to develop a greater interest in the venom ontogenetic variability of this species, which is little explored so far. Among snakes of the genus Bothrops, B. jararacussu was previously described as the one with highest myotoxic activity. Another peculiarity was also observed in its venom: a low rate of immunogenicity. In addition, its activity is not efficiently neutralized by the specific antibothropic serum. Considering these particularities, we performed an ontogenetic study of B. jararacussu using venom samples from newborns of the same litter (<6 months) and adults (>24 months). Our results identified two distinct profiles in the venom of these animals: young individuals with little PLA(2) K-49 and more proteases; and adults with a lot of the same myotoxic PLA(2), but less proteases. The HPLC and SDS-PAGE profiles corroborated our findings. Adults showed more hemorrhagic activity in vivo than juveniles, while adult males showed less activity when compared to females. In vivo myotoxicity activity was higher in adults than in juveniles. Immune recognition assays showed different results for the distinct venom. (AU) | |
| Processo FAPESP: | 17/16908-2 - Caracterização proteômica e das atividades enzimáticas e fisiopatológicas do veneno das espécies de serpentes que compõem o grupo Bothrops neuwiedi |
| Beneficiário: | Karen de Morais Zani |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/25786-0 - Estudo dos inibidores de fosfolipase A2 (PLIs) presentes nos plasmas das serpentes peçonhentas e não-peçonhentas e avaliação da atividade neutralizante destes sobre as atividades da fosfolipase A2 (PLA2) dos venenos de serpentes |
| Beneficiário: | Anita Mitico Tanaka-Azevedo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/14724-4 - Caracterização proteômica e funcional do veneno de duas espécies de serpentes do gênero Bothrops submetidas à mudança de dieta |
| Beneficiário: | Nathália da Costa Galizio |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 18/20651-0 - Análise da variabilidade ontogenética e sazonal do veneno da serpente Bothrops jararacussu (Serpentes: Viperidae; Lacerda 1884) |
| Beneficiário: | Weslei da Silva Aguiar |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |