In situ study of cellular immune response in human cutaneous lesions caused by Leishmania (Viannia) panamensis in Panama

Autor(es)

Gonzalez, Kadir ^{[1, 2]} ; Calzada, Jose Eduardo ^{[1, 3]} ; Tomokane, Thaise Yumie ^[2] ; Sandoval Pacheco, Carmen Maria ^[2] ; Araujo Flores, Gabriela Venicia ^[2] ; Castro Gomes, Claudia Maria ^[2] ; Pereira Corbett, Carlos Eduardo ^[2] ; Saldana, Azael ^{[1, 4]} ; Laurenti, Marcia Dalastra ^[2]

Afiliação do(s) autor(es)

^[1] Inst Conmemorat Gorgas Estudios Salud, Dept Parasitol Mol, Panama City - Panama

^[2] Univ Sao Paulo, Fac Med, Dept Patol, Lab Patol Molestias Infecciosas, Sao Paulo - Brazil

^[3] Univ Panama, Fac Med Vet, Panama City - Panama

^[4] Univ Panama, Fac Med, Ctr Invest & Diagnost Enfermedades Parasitarias, Panama City - Panama

• Tipo de documento: Artigo Científico
• Fonte: PARASITE IMMUNOLOGY; v. 43, n. 3 OCT 2020.
• Citações Web of Science: 0

Resumo

Aims: Leishmaniasis is considered a disease with multiple clinical/immunopathological characteristics, depending on the immunity of the host and the species of the parasite. In Panama, the most prevalent species that causes localized cutaneous leishmaniasis (LCL) is Leishmania (Viannia) panamensis, and its immune response is poorly studied. Therefore, we evaluated by immunohistochemistry, the in situ immune response during this infection. Methods and Results: Biopsies from Panamanian patients with LCL were collected and processed by histological techniques. Infection by L. (V.) panamensis was demonstrated by isolation in culture and molecular characterization by Hsp70-RFLP. The in situ immune response was assessed by immunohistochemistry. The immune response was characterized by predominance of T cells, mainly CD8 cells that showed positive correlation with IFN-gamma and Granzyme B. CD4 cells presented positive correlation with both IFN-gamma and IL-13, pointed by mixed cellular immune response. Regulatory response was characterized by FoxP3 cells, which showed positive correlation to IL-10 but not with TGF-beta. Conclusions: L. (V.) panamensis infection triggers a mixed cellular immune response, characterized by the presence of pro-inflammatory, anti-inflammatory and regulatory elements in the skin lesion of Panamanian patients. These data contribute to a better understanding of the immunopathogenesis of Leishmania Viannia infection in Panama. (AU)

• Processo FAPESP: 17/03141-5 - Caracterização das alterações histopatológicas e da resposta imune celular em lesões cutâneas de pacientes com Leishmaniose tegumentar no Panamá
• Beneficiário: Kadir Amilcar Gonzalez Carrion
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 14/50315-0 - Leishmanioses na América Latina: uma perspectiva avançada sobre fatores imunopatogenéticos da infecção cutânea e visceral, imunomoduladores da saliva de vetores flebotomíneos e exo-antígenos imunogênicos de Leishmania (L.) infantum chagasi candidatos à vacina
• Beneficiário: Marcia Dalastra Laurenti
• Modalidade de apoio: Auxílio à Pesquisa - Temático