Allograft Inflammatory Factor AIF-1: early immune response in the Mediterranean sea urchin Paracentrotus lividus

Autor(es)

Chiaramonte, Marco ^[1] ; Arizza, Vincenzo ^[1] ; La Rosa, Simona ^[1] ; Queiroz, Vinicius ^{[1, 2]} ; Mauro, Manuela ^[1] ; Vazzana, Mirella ^[1] ; Inguglia, Luigi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Palermo, Dept STEBICEF, Via Archirafi 18, I-90123 Palermo - Italy

^[2] Univ Sao Paulo, Inst Biosci, Dept Physiol, Rua Matao, Travessa 14, 101, BR-05508090 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZOOLOGY; v. 142, OCT 2020.
• Citações Web of Science: 1

Resumo

Echinoderms are a phylum of deuterostomic invertebrates that play a key role in maintaining the biodiversity of marine ecosystems. They represent a good study model for immunity because their coelomic fluid contains different types of cells involved in the inflammatory response: the coelomocytes. In the case of the sea urchin Paracentrotus lividus, the coelomocyte population is mainly represented by amoebocytes and uncoloured spherulocytes that implement a defence program through phagocytosis, encapsulation, cytotoxicity, and production of antimicrobial agents. The purpose of this study was to find evidence of a possible modulating effect of lipopolysaccharide LPS on the expression of the AIF-1 gene and therefore to determine whether or not there is a correlation between the P. lividus immune response and the expression of this gene when the homeostasis of the animals is disturbed by a bacterial infection which, in this case, was simulated with treatment with LPS. AIF-1 (Allograft Inflammatory Factor 1) is a 17 kDa calcium-binding protein that, in vertebrates, is involved in the activation of macrophages. Similarly, in Echinoids and in general in invertebrates, the expression of this gene increases considerably after a bacterial attack and this suggests that it plays a key role during the immune responses. (AU)

• Processo FAPESP: 15/21460-5 - Função fisiológica, composição química e ontogenia dos esferulócitos em Echinoidea (Echinodermata).
• Beneficiário: Vinicius Queiroz Araújo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/14497-8 - Função fisiológica e origem dos esferulócitos no ouriço-do-mar Paracentrotus lividus (Echinodermata: Echinoidea)
• Beneficiário: Vinicius Queiroz Araújo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado